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Tropic effects of otic epithelium on cochleo-vestibular ganglion fiber growth in vitro
- Source :
- The Anatomical Record. 232:273-284
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- Sensory nerve fibers of the cochleo-vestibular ganglion (CVG) innervate the otic epithelium in the early chick embryo by directed growth. To see if the target tissue could exert a tropic influence, we co-cultured CVGs from chick embryos (Hamburger-Hamilton stages 16-30) in a 3D collagen matrix with their normal target epithelium or with other epithelial tissues taken from the same or different stages of development. The pattern of neurite outgrowth and the viability of the CVG after five days in vitro were assessed histologically with a silver method. On the basis of the patterns of neurite outgrowth directed toward the epithelium, the cultures were classified as having slightly, mostly, exclusively, or no directed outgrowth. Of 49 cultures containing otic epithelium, 33 had mostly or exclusively directed growth patterns. This effect did not depend on any particular stage difference between co-cultures or on their viability in vitro. Cultures of non-sensory otic epithelium (endolymphatic duct) also presented directed growth patterns. Co-cultures with ectoderm from forelimb or visceral arch had little, if any, directed growth. The directed growth could not be explained simply as a result of guidance by non-neuronal cells or of the viability of the explants. The results are consistent with the hypothesis that the otic epithelium provides a tropic factor that attracts growing CVG fibers.
- Subjects :
- Pathology
medicine.medical_specialty
animal structures
Neurite
Scarpa's ganglion
Ectoderm
Cell Communication
Chick Embryo
In Vitro Techniques
Vestibular Nerve
Biology
Epithelium
Endolymphatic duct
medicine
Animals
Nerve Growth Factors
Cochlear Nerve
Cells, Cultured
Epithelial Cells
Embryo
Agricultural and Biological Sciences (miscellaneous)
Ganglion
Cell biology
medicine.anatomical_structure
Ear, Inner
embryonic structures
Anatomy
Sensory nerve
Subjects
Details
- ISSN :
- 10970185 and 0003276X
- Volume :
- 232
- Database :
- OpenAIRE
- Journal :
- The Anatomical Record
- Accession number :
- edsair.doi.dedup.....9ff9eec214c758c5764e6c0e39bcb93d