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MicroRNA-127-5p regulates osteopontin expression and osteopontin-mediated proliferation of human chondrocytes
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- The aim of this study was to determine the specific microRNA (miRNA) that regulates expression of osteopontin (OPN) in osteoarthritis (OA). The potential regulatory miRNAs for OPN messenger RNA (mRNA) were predicted by miRNA prediction programs. Among eight potential regulatory miRNAs, miR-220b, miR-513a-3p and miR-548n increased, while miR-181a, miR-181b, miR-181c, miR-181d and miR-127-5p decreased in OA patients. miRNA-127-5p mimics suppressed OPN production as well as the activity of a reporter construct containing the 3′-UTR of human OPN mRNA. In addition, mutation of miR-127-5p binding site in the 3′-UTR of OPN mRNA abolished miR-127-5p-mediated repression of reporter activity. Conversely, treatment with miR-127-5p inhibitor increased reporter activity and OPN production. Interestingly, miR-127-5p inhibited proliferation of chondrocytes through OPN. In conclusion, miRNA-127-5p is an important regulator of OPN in human chondrocytes and may contribute to the development of OA.
- Subjects :
- 0301 basic medicine
Untranslated region
Down-Regulation
Biology
Article
03 medical and health sciences
Chondrocytes
Downregulation and upregulation
stomatognathic system
microRNA
Osteoarthritis
Humans
Osteopontin
Psychological repression
3' Untranslated Regions
Cell Proliferation
Regulation of gene expression
Messenger RNA
Multidisciplinary
Binding Sites
Three prime untranslated region
Cell biology
MicroRNAs
030104 developmental biology
Gene Expression Regulation
Immunology
Mutation
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....9ff48df9c6ef5c089a1a802c10cd8fdc
- Full Text :
- https://doi.org/10.1038/srep25032