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Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrix
- Source :
- PLoS ONE, Vol 16, Iss 3, p e0247567 (2021), PLoS ONE
- Publication Year :
- 2021
-
Abstract
- Despite the increasing demand of cellular therapies for dogs, little is known on the differences between adult and fetal adnexa canine mesenchymal stem cells (MSCs), and data on their metabolic features are lacking. The present study aimed at comparing the characteristics of canine adipose tissue (AT) and umbilical cord matrix (UC) MSCs. Moreover, for the first time in the dog, the cellular bioenergetics were investigated by evaluating the two main metabolic pathways (oxidative phosphorylation and glycolysis) of ATP production. Frozen-thawed samples were used for this study. No differences in mean cell proliferation were found (P>0.05). However, while AT-MSCs showed a progressive increase in doubling time over passages, UC-MSCs showed an initial post freezing-thawing latency. No differences in migration, spheroid formation ability, and differentiation potential were found (P>0.05). RT-PCR analysis confirmed the expression of CD90 and CD44, the lack of CD14 and weak expression of CD34, mostly by AT-MSCs. DLA-DRA1 and DLA-DQA1 were weakly expressed only at passage 0 by UC-MSCs, while they were expressed at different passages for AT-MSCs. There was no difference (P>0.05) in total ATP production between cell cultures, but the ratio between the “mitochondrial ATP Production Rate” and the “glycolytic ATP Production Rate” was higher (P
- Subjects :
- Bioenergetics
Cellular differentiation
Gene Expression
Adipose tissue
Antigens, CD34
Cell Separation
Mitochondrion
Biochemistry
Umbilical Cord
Canine, mesenchymal stem cells, adipose tissue, Foetal adnexa
Major Histocompatibility Complex
Adenosine Triphosphate
Animal Cells
Cell Movement
Medicine and Health Sciences
Glycolysis
Energy-Producing Organelles
Cells, Cultured
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Stem Cells
Stem Cell Therapy
Cell Differentiation
Mitochondria
Hyaluronan Receptors
Adipose Tissue
Connective Tissue
Medicine
Biological Cultures
Cellular Types
Cellular Structures and Organelles
Anatomy
Research Article
Cell Physiology
Science
Oxidative phosphorylation
Research and Analysis Methods
Andrology
Dogs
Animals
Metabolomics
Cell Proliferation
Clinical Genetics
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
Cell Cultures
Cell Metabolism
Transplantation
Biological Tissue
Thy-1 Antigens
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Vol 16, Iss 3, p e0247567 (2021), PLoS ONE
- Accession number :
- edsair.doi.dedup.....9ff258747d5e4f7e60be000d671dead5