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Biomechanical and proteomic analysis of INF- β-treated astrocytes
- Source :
- Nanotechnology. 20:455106
- Publication Year :
- 2009
- Publisher :
- IOP Publishing, 2009.
-
Abstract
- Astrocytes have a key role in the pathogenesis of several diseases including multiple sclerosis and were proposed as the designed target for immunotherapy. In this study we used atomic force microscopy (AFM) and proteomics methods to analyse and correlate the modifications induced in the viscoleastic properties of astrocytes to the changes induced in protein expression after interferon- beta (IFN-beta) treatment. Our results indicated that IFN-beta treatment resulted in a significant decrease in the Young's modulus, a measure of cell elasticity, in comparison with control cells. The molecular mechanisms that trigger these changes were investigated by 2DE (two-dimensional electrophoresis) and confocal analyses and confirmed by western blotting. Altered proteins were found to be involved in cytoskeleton organization and other important physiological processes.
- Subjects :
- Proteomics
Materials science
Cytoskeleton organization
medicine.medical_treatment
Confocal
Blotting, Western
Cell
Bioengineering
Microscopy, Atomic Force
Nanoindentation
Pathogenesis
Atomic force microscopy
Mice
Interferon
medicine
Animals
Electrophoresis, Gel, Two-Dimensional
General Materials Science
Electrical and Electronic Engineering
Cells, Cultured
Cytoskeleton
Microscopy, Confocal
Mechanical Engineering
Multiple sclerosis
Interferon-beta
General Chemistry
Immunotherapy
medicine.disease
Molecular biology
Biomechanical Phenomena
Blot
medicine.anatomical_structure
Scanning probe microscopy
Mechanics of Materials
Astrocytes
medicine.drug
Subjects
Details
- ISSN :
- 13616528 and 09574484
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Nanotechnology
- Accession number :
- edsair.doi.dedup.....9fe0c0fd2b73deec64c36f8c96bfc6d0