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Long-lived Memory T Lymphocyte Responses After Hantavirus Infection
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2002
- Publisher :
- The Rockefeller University Press, 2002.
-
Abstract
- Puumala virus (PUUV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS), which is an important public health problem in large parts of Europe. We examined the memory cytolytic T lymphocyte (CTL) responses in 13 Finnish individuals who had HFRS between 1984 and 1995. In seven of these donors, we detected virus-specific CTL responses against the PUUV nucleocapsid (N) protein after in vitro stimulation with PUUV. Six novel CD8+ CTL epitopes were defined on the N protein and were found to be restricted by various HLA alleles including A2, A28, B7, and B8. This is the first demonstration of PUUV-specific CTL responses in humans, and the first identification of CTL epitopes on PUUV. In addition, this study provides one of the few characterizations of a human antiviral memory T cell response, without the complicating issues of virus persistence or reinfection. Interferon (IFN)-γ ELISPOT analysis showed that memory CTL specific for these epitopes were present at high frequency in PUUV-immune individuals many years after acute infection in the absence of detectable viral RNA. The frequencies of PUUV-specific CTL were comparable to or exceeded those found in other viral systems including influenza, EBV and HIV, in which CTL responses may be boosted by periodic reinfection or virus persistence.
- Subjects :
- Time Factors
T lymphocyte epitopes
Immunology
Molecular Sequence Data
chemical and pharmacologic phenomena
In Vitro Techniques
cytotoxic T lymphocytes
Puumala virus
Epitope
Article
hantavirus
Epitopes
medicine
Immunology and Allergy
Cytotoxic T cell
Humans
Amino Acid Sequence
Nucleocapsid
Antigens, Viral
Finland
Hantavirus
immunologic memory
biology
ELISPOT
Nucleocapsid Proteins
biology.organism_classification
Virology
CTL
medicine.anatomical_structure
Hemorrhagic Fever with Renal Syndrome
Hantavirus Infection
Memory T cell
T-Lymphocytes, Cytotoxic
Subjects
Details
- Language :
- English
- ISSN :
- 15409538 and 00221007
- Volume :
- 196
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....9fcf2f5fcdf59d8e64de334373af1e9a