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Effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos
- Source :
- Molecular reproduction and development. 61(4)
- Publication Year :
- 2002
-
Abstract
- The present study examined the effect of elevated Ca2+ concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion and activation were induced simultaneously with electric pulses in 0.3 M mannitol-based medium containing 0.1 or 1.0 mM CaCl2. Some fused embryos were further activated 1 hr after the fusion treatment by exposure to an electric pulse. The NT embryos were cultured in vitro for 6 days. Fusion and blastocyst formation rates were significantly (P
- Subjects :
- Swine
Cloning, Organism
Perivitelline space
Biology
Andrology
Cell Fusion
Mice
Genetics
medicine
Animals
Blastocyst
Fibroblast
Cell fusion
Embryogenesis
Genetic transfer
Embryo culture
Cell Biology
Fibroblasts
Embryo Transfer
Culture Media
medicine.anatomical_structure
embryonic structures
Immunology
Oocytes
Somatic cell nuclear transfer
Calcium
Female
Developmental Biology
Subjects
Details
- ISSN :
- 1040452X
- Volume :
- 61
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.doi.dedup.....9fc54b2ee8cce645ba42915f9d7cba4d