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Identification of characteristic compounds of moderate volatility in breast cancer cell lines
- Source :
- PLoS ONE, Vol 15, Iss 6, p e0235442 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.
- Subjects :
- Nonanal
Cell Lines
Biochemistry
Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Spectrum Analysis Techniques
0302 clinical medicine
Breast Tumors
Tumor Cells, Cultured
Medicine and Health Sciences
Extraction Techniques
0303 health sciences
Multidisciplinary
Fatty Acids
Chromatographic Techniques
Lipids
Laboratory Equipment
Chemistry
Oncology
030220 oncology & carcinogenesis
Physical Sciences
Engineering and Technology
Medicine
Female
Biological Cultures
Oxidation-Reduction
Decenoic Acid
Research Article
Science
Equipment
Breast Neoplasms
Research and Analysis Methods
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
Breast cancer
Diagnostic Medicine
Breast Cancer
Cancer Detection and Diagnosis
medicine
Humans
Solid-Phase Extraction
Solid Phase Microextraction
030304 developmental biology
Volatile Organic Compounds
Chromatography
Cancers and Neoplasms
Biology and Life Sciences
Cancer
Cell Cultures
Decanal
medicine.disease
Culture Media
Heptanal
Octanal
chemistry
Cultured Fibroblasts
Gas chromatography–mass spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....9fbd9f38714ec962c0bff0504ad5512e