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The S100A10-AnxA2 complex is associated with the exocytosis of hepatitis B virus in intrauterine infection
- Source :
- Laboratory Investigation; a Journal of Technical Methods and Pathology
- Publication Year :
- 2021
-
Abstract
- Mother-to-child transmission (MTCT) is the major cause of chronic infection of hepatitis B virus (HBV) in patients. However, whether and how HBV crosses the placenta to cause infection in utero remains unclear. In this study, we investigate the mechanism as to how the HBV virions pass through layers of the trophoblast. Our data demonstrate the exocytosis of virions from the trophoblast after exposure to HBV where the endocytosed HBV virions co-localized with an S100A10/AnxA2 complex and LC3, an autophagosome membrane marker. Knockdown of either AnxA2 or S100A10 in trophoblast cells led to a reduction of the amount of exo-virus in Transwell assay. Immunohistochemistry also showed a high expression of AnxA2 and S100A10 in the placental tissue samples of HBV-infected mothers with congenital HBV-positive infants (HBV+/+). We conclude that in HBV intrauterine infection and mother-to-child transmission, a proportion of HBV hijacks autophagic protein secretion pathway and translocate across the trophoblast via S100A10/AnxA2 complex and multivesicular body (MVB)-mediated exocytosis. Our study provides a potential target for the interference of the mechanisms of HBV intrauterine infection and mother-to-child transmission.<br />Mother-to-child transmission is the major cause of chronic hepatitis B virus (HBV) infection. This study shows that an unconventional protein secretion pathway that depends on autophagy may be hijacked by HBV to complete the process of intracellular transport. In HBV-infected trophoblasts, AnxA2-S100A10-mediated exocytosis may result in HBV intrauterine transmission.
- Subjects :
- Hepatitis B virus
Placenta
medicine.disease_cause
Exocytosis
Article
Pathology and Forensic Medicine
Cell Line
Microscopy, Electron, Transmission
Pregnancy
medicine
Humans
Molecular Biology
Annexin A2
Cells, Cultured
biology
S100 Proteins
Uterus
S100A10
Infant, Newborn
Trophoblast
virus diseases
Cell Biology
Hepatitis B
Virology
digestive system diseases
Infectious Disease Transmission, Vertical
Trophoblasts
Chronic infection
Secretory protein
medicine.anatomical_structure
Multiprotein Complexes
Autophagosome membrane
embryonic structures
biology.protein
Female
Infection
Subjects
Details
- ISSN :
- 15300307
- Volume :
- 102
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Laboratory investigation; a journal of technical methods and pathology
- Accession number :
- edsair.doi.dedup.....9fb86a1686922f0e99f074059c521120