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Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars
- Source :
- Analytical and Bioanalytical Chemistry. 410:863-868
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Lateral flow type detection is becoming interesting not only in regions with a poor medical infrastructure but also for practitioners in day-to-day clinical work or for veterinary control in case of possible epidemics. In this work, we describe the first steps of development of a multi-channel strip with potential internal calibration of multiparametric and colorimetric lateral flow assays for the simultaneous detection of the lipopolysaccharides (LPS) of Salmonella typhimurium (S. typhimurium) and Salmonella enteritidis (S. enteritidis). We structured four channels in the nitrocellulose membrane with a Yb:KGW solid-state femtosecond laser ("cold" ablation process) to form distinct tracks of porous material and used gold nanoparticles for the labeling of the antibodies. In addition, calibration curves of the spot intensities of both serovars are presented, and it was shown that no cross reactivity between the different capture antibodies and LPS occurred. Finally, we detected LPS of both Salmonella serovars simultaneously. The color changes (spot intensities of the reaction zones) were evaluated using the open-source image-processing program ImageJ. Graphical abstract Multiparametric testing, strip A was tested with LPS S. enteritidis ( c=0.01 g/L) and LPS S.typhimurium ( c=0.0001 g/L), strip B with LPS S. enteritidis ( c=0.001 g/L) and LPS S. typhimurium ( c=0.001g/L) and strip C with LPS S. enteritidis (c=0.0001 g/L) and LPS S. typhimurium ( c=0.01 g/L), and read-out.
- Subjects :
- Lipopolysaccharides
Paper
Salmonella typhimurium
Serotype
Salmonella
Point-of-Care Systems
Salmonella enteritidis
Analytical chemistry
02 engineering and technology
medicine.disease_cause
01 natural sciences
Biochemistry
Cross-reactivity
Analytical Chemistry
Clinical work
medicine
Humans
Immunoassay
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Collodion
Membranes, Artificial
Equipment Design
Paper based
021001 nanoscience & nanotechnology
Molecular biology
0104 chemical sciences
Salmonella Infections
Colorimetry
0210 nano-technology
Lateral flow immunoassay
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 410
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....9fb1e1423d23dccf2e24f1479d3cbef1