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Synthesis of Bacillithiol and the Catalytic Selectivity of FosB-type Fosfomycin Resistance Proteins
Synthesis of Bacillithiol and the Catalytic Selectivity of FosB-type Fosfomycin Resistance Proteins
- Publication Year :
- 2012
-
Abstract
- Bacillithiol (BSH) has been prepared on the gram scale from the inexpensive starting material, d-glucosamine hydrochloride, in 11 steps and 8–9% overall yield. The BSH was used to survey the substrate and metal-ion selectivity of FosB enzymes from four Gram-positive microorganisms associated with the deactivation of the antibiotic fosfomycin. The in vitro results indicate that the preferred thiol substrate and metal ion for the FosB from Staphylococcus aureus are BSH and Ni(II), respectively. However, the metal-ion selectivity is less distinct with FosB from Bacillus subtilis, Bacillus anthracis, or Bacillus cereus.
- Subjects :
- Staphylococcus aureus
Stereochemistry
Bacillus cereus
Bacillus
Bacillus subtilis
Fosfomycin
Biochemistry
Article
Substrate Specificity
chemistry.chemical_compound
medicine
Cysteine
Physical and Theoretical Chemistry
Glucosamine
biology
Molecular Structure
Chemistry
fungi
Organic Chemistry
Substrate (chemistry)
biology.organism_classification
Bacillus anthracis
Bacillithiol
Biocatalysis
Proto-Oncogene Proteins c-fos
FOSB
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9f8f01ce1d696fd8fb27b5e7370971ed