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Paramecium putrinum (Ciliophora, Protozoa): The first insight into the variation of two DNA fragments – Molecular support for the existence of cryptic species
- Source :
- Molecular Phylogenetics and Evolution. 73:140-145
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Paramecium putrinum (Claparede & Lachmann 1858) is one of the smallest (80–140 μm long) species of the genus Paramecium . Although it commonly occurs in freshwater reservoirs, no molecular studies of P. putrinum have been conducted to date. Herein we present an assessment of molecular variation in 27 strains collected from widely separated populations by using two selected DNA fragments (ITS1-5.8S-ITS2-5′LSU rDNA and COI mtDNA). Both the trees and haplotype networks reconstructed for both genome fragments show that the studied strains of P. putrinum form five main haplogroups. The mean distance between the studied strains is p -distance = 0.007/0.068 (rDNA/ COI ) and exhibits similar variability as that between P. bursaria syngens . Based on these data, one could hypothesize that the clusters revealed in the present study may correspond to previously reported syngens and that there are at least five cryptic species within P. putrinum .
- Subjects :
- Genetics
Mitochondrial DNA
Species complex
Genome
Paramecium
Polymorphism, Genetic
biology
Haplotype
Sequence Analysis, DNA
Bursaria
biology.organism_classification
DNA, Mitochondrial
DNA, Ribosomal
Haplogroup
Haplotypes
Genus
DNA Barcoding, Taxonomic
Molecular Biology
Phylogeny
Ecology, Evolution, Behavior and Systematics
Subjects
Details
- ISSN :
- 10557903
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Molecular Phylogenetics and Evolution
- Accession number :
- edsair.doi.dedup.....9f816569e90da2c18bab5fa9fea47d6f