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Genome size and organization in the blacklegged tick, Ixodes scapularis and the Southern cattle tick, Boophilus microplus
- Source :
- Insect molecular biology. 14(2)
- Publication Year :
- 2005
-
Abstract
- Genome sizes and the organization of repetitive DNA were determined in the hard ticks Ixodes scapularis and Boophilus microplus using reassociation kinetics. The I. scapularis genome contains approximately 2.15 pg (2.1x10(3) Mbp) of DNA and consists of no foldback (FB), 27% highly repetitive (HR), 39% moderately repetitive (MR), and 34% unique DNA. The B. microplus genome contains 7.5 pg (7.1x10(3) Mbp) DNA, and consists of 0.82% FB, 31% HR, 38% MR, and 30% unique DNA. In both species, repetitive sequences occur in a mixture of long and short period interspersion but most (65-80%) of the DNA follows a pattern of short period interspersion. Genome size and organization in the three tick species so far examined are distinct from other arthropods in having a greater proportion of MR, a lower proportion of unique and HR DNA of very low sequence complexity.
- Subjects :
- Ixodidae
Period (gene)
Biology
Tick
Genome
chemistry.chemical_compound
Genome Components
Species Specificity
Genetics
Animals
Repeated sequence
Molecular Biology
Genome size
Repetitive Sequences, Nucleic Acid
Chromatography
Foldback (sound engineering)
DNA
biology.organism_classification
Virology
Molecular biology
Kinetics
chemistry
Ixodes scapularis
Insect Science
Nucleic Acid Renaturation
Regression Analysis
Subjects
Details
- ISSN :
- 09621075
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Insect molecular biology
- Accession number :
- edsair.doi.dedup.....9f8125875f4deab30501781a2fc366c7