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UVB-Induced Inflammatory Cytokine Release, DNA Damage and Apoptosis of Human Oral Compared with Skin Tissue Equivalents
- Source :
- Photochemistry and Photobiology. 89:665-670
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- People can get oral cancers from UV (290-400 nm) exposures. Besides high outdoor UV exposures, high indoor UV exposures to oral tissues can occur when consumers use UV-emitting tanning devices to either tan or whiten their teeth. We compared the carcinogenic risks of skin to oral tissue cells after UVB (290-320 nm) exposures using commercially available 3D-engineered models for human skin (EpiDerm™), gingival (EpiGing™) and oral (EpiOral™) tissues. To compare the relative carcinogenic risks, we investigated the release of cytokines, initial DNA damage in the form of cyclobutane pyrimidine dimers (CPDs), repair of CPDs and apoptotic cell numbers. We measured cytokine release using cytometric beads with flow cytometry and previously developed a fluorescent immunohistochemical assay to quantify simultaneously CPD repair rates and apoptotic cell numbers. We found that interleukin-8 (IL-8) release and the initial CPDs are significantly higher, whereas the CPD repair rates and apoptotic cell numbers are significantly lower for oral compared with skin tissue cells. Thus, the increased release of the inflammatory cytokine IL-8 along with inefficient CPD repair and decreased death rates for oral compared with skin tissue cells suggests that mutations are accumulating in the surviving population of oral cells increasing people's risks for getting oral cancers.
- Subjects :
- Skin Neoplasms
DNA Repair
Ultraviolet Rays
DNA damage
medicine.medical_treatment
Population
Cell
Apoptosis
Human skin
Pyrimidine dimer
Biology
Biochemistry
Flow cytometry
Cell Line, Tumor
medicine
Humans
Physical and Theoretical Chemistry
education
Skin
education.field_of_study
integumentary system
medicine.diagnostic_test
Interleukin-8
Mouth Mucosa
Dose-Response Relationship, Radiation
General Medicine
Cytokine
medicine.anatomical_structure
Organ Specificity
Pyrimidine Dimers
Immunology
Cancer research
Mouth Neoplasms
DNA Damage
Subjects
Details
- ISSN :
- 00318655
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Photochemistry and Photobiology
- Accession number :
- edsair.doi.dedup.....9f4e3b15176f9acc1c3890d58e00a071