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Isolation and characterization of three new monoterpene synthases from Artemisia annua
- Source :
- Frontiers in Plant Science, Vol 7 (2016), Frontiers in Plant Science
- Publication Year :
- 2016
- Publisher :
- Frontiers Media S.A., 2016.
-
Abstract
- Artemisia annua, an annual herb used in traditional Chinese medicine, produces a wealth of monoterpenes and sesquiterpenes, including the well-known sesquiterpene lactone artemisinin, an active ingredient in the treatment for malaria. Here we report three new monoterpene synthases of A. annua. From a glandular trichome cDNA library, monoterpene synthases of AaTPS2, AaTPS5 and AaTPS6, were isolated and characterized. The recombinant proteins of AaTPS5 and AaTPS6 produced multiple products with camphene and 1,8-cineole as major products, respectively, and AaTPS2 produced a single product, β-myrcene. Although both Mg2+ and Mn2+ were able to support their catalytic activities, altered product spectrum was observed in the presence of Mn2+ for AaTPS2 and AaTPS5. Analysis of extracts of aerial tissues and root of A. annua with gas chromatography-mass spectrometry (GC-MS) detected more than 20 monoterpenes, of which the three enzymes constituted more than 1/3 of the total. Mechanical wounding induced the expression of all three monoterpene synthase genes, and transcript levels of AaTPS5 and AaTPS6 were also elevated after treatments with phytohormones of methyl jasmonate (MeJA), salicylic acid (SA) and gibberellin (GA), suggesting a role of these monoterpene synthases in plant-environment interactions. The three new monoterpene synthases reported here further our understanding of molecular basis of monoterpene biosynthesis and regulation in plant.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Monoterpene
Artemisia annua
Plant Science
lcsh:Plant culture
Sesquiterpene lactone
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Monoterpene synthase
Botany
8-cineole
lcsh:SB1-1110
Cineole
Original Research
chemistry.chemical_classification
Methyl jasmonate
biology
camphene
biology.organism_classification
Trichome
030104 developmental biology
chemistry
Biochemistry
Camphene
Gibberellin
β-myrcene
Salicylic acid
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....9f4c232dfc9c0b9e5d070465765a279d