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Quantitative microarray-based DNA-DNA hybridization assay for measuring genetic distances among bacterial species and its application to the identification of family Enterobacteriaceae
- Source :
- Microbiology and immunology. 49(3)
- Publication Year :
- 2005
-
Abstract
- Quantitative DNA-DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3-labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis. These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA-DNA hybridization for measuring chromosome relatedness among bacterial species.
- Subjects :
- DNA, Bacterial
Escherichia
Serratia
Time Factors
Microarray
Immunology
Microbiology
Bacterial genetics
Nucleic acid thermodynamics
chemistry.chemical_compound
Enterobacteriaceae
Species Specificity
Salmonella
Virology
Klebsiella
Yersinia pseudotuberculosis
Oligonucleotide Array Sequence Analysis
Genetics
biology
DNA–DNA hybridization
Temperature
Chromosome
Nucleic Acid Hybridization
biology.organism_classification
Molecular biology
chemistry
Shigella
DNA
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Microbiology and immunology
- Accession number :
- edsair.doi.dedup.....9f4433df6b5107e018778723cc2c15d3