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Does Osmotic Stress Affect Natural Product Expression in Fungi?
- Source :
- Marine drugs, Marine drugs, MDPI, 2017, 15 (8), pp.254. ⟨10.3390/md15080254⟩, Marine Drugs; Volume 15; Issue 8; Pages: 254, Marine Drugs, Vol 15, Iss 8, p 254 (2017), Marine Drugs
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- The discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. An unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawater contributes to enhanced secondary metabolism? To answer this question, a terrestrial isolate of Aspergillus aculeatus was fermented in osmotic and saline stress conditions in parallel across multiple sites. The ex-type strain of A. aculeatus was obtained from three different culture collections. Site-to-site variations in metabolite expression were observed, suggesting that subculturing of the same strain and subtle variations in experimental protocols can have pronounced effects upon metabolite expression. Replicated experiments at individual sites indicated that secondary metabolite production was divergent between osmotic and saline treatments. Titers of some metabolites increased or decreased in response to increasing osmolite (salt or glycerol) concentrations. Furthermore, in some cases, the expression of some secondary metabolites in relation to osmotic and saline stress was attributed to specific sources of the ex-type strains.
- Subjects :
- 0301 basic medicine
fungi
metabolite expression
LC-MS
metabolome
osmotic stress
Osmotic shock
[SDV]Life Sciences [q-bio]
Metabolite
Secondary Metabolism
Pharmaceutical Science
Marine Biology
Sodium Chloride
Secondary metabolite
01 natural sciences
Article
03 medical and health sciences
chemistry.chemical_compound
Osmotic Pressure
Drug Discovery
medicine
Metabolome
[CHIM]Chemical Sciences
Seawater
Secondary metabolism
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
ComputingMilieux_MISCELLANEOUS
Biological Products
Molecular Structure
Strain (chemistry)
biology
010405 organic chemistry
Aspergillus aculeatus
biology.organism_classification
Anti-Bacterial Agents
0104 chemical sciences
Aspergillus
030104 developmental biology
lcsh:Biology (General)
Biochemistry
chemistry
Fermentation
medicine.drug
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....9f3aa694a7e8ef200cebc80766f08536