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MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer
- Source :
- Bioscience reports. 39(12)
- Publication Year :
- 2019
-
Abstract
- Colorectal cancer (CRC) remains the candidate for one of the typical types of malignant tumors of in gastrointestinal tract all around the world, which leads to tremendous death and ranks as the top leading death of cancer. Recently, microRNAs have emerged as double-edged sword in numerous cancers. This investigation aims to discuss the regulative role of microRNA-574-3p (miR-574-3p), elucidating its molecular mechanism and clinical significance in CRC. Herein, it revealed to us that miR-574-3p was lowly expressed in CRC tissues in comparison with the matched paracarcinoma tissues. In addition, transfection of SW480 and HT29 cells with miR-574-3p mimics prohibited the post-transcriptional expression of Cyclin D2 (CCND2), which then significantly blocked cell growth and cell migration, yet triggered cell apoptosis. Also, dual-luciferase reporter assays proved the role of CCND2 as the targeted gene for miR-574-3p. miR-574-3p overexpression prohibited the activity of CCND2 in SW480 and HT29 cells. Silencing of CCND2 in SW480 and HT29 CRC cell lines leading to reduced cell proliferative and migrative rates, and enhanced apoptotic rate. The suppressive effects of elevation of miR-574-3p on the proliferation of the human CRC cells and promotive effects on cell apoptosis by targeting CCND2 were further illustrated in the in vitro studies. Thus, we hypothesize that miR-574-3p may be served as a prospective therapeutic candidate for CRC.
- Subjects :
- 0301 basic medicine
Male
Colorectal cancer
Cell
Biophysics
Apoptosis
Biology
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cyclin D2
Cell Movement
microRNA
medicine
Gene silencing
Humans
Neoplasm Invasiveness
neoplasms
Molecular Biology
Aged
Cell Proliferation
Cell growth
Cancer
Cell Biology
Transfection
Middle Aged
medicine.disease
HCT116 Cells
digestive system diseases
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer research
Disease Progression
Female
Colorectal Neoplasms
HT29 Cells
Subjects
Details
- ISSN :
- 15734935
- Volume :
- 39
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Bioscience reports
- Accession number :
- edsair.doi.dedup.....9f33e7d2a83c2e6392a587bb25abd785