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EFFECT OF STORAGE TIME AND STORAGE CONDITIONS ON ANTIBODY DETECTION IN BLOOD SAMPLES COLLECTED ON FILTER PAPER
- Source :
- Journal of wildlife diseases. 52(3)
- Publication Year :
- 2016
-
Abstract
- Using filter paper to collect blood from wildlife for antibody analysis can be a powerful technique to simplify the collection, transport, and storage of blood samples. Despite these advantages, there are limited data that detail how long these samples can be stored and how storage conditions affect antibody longevity. We used blood samples collected on filter paper from coyotes experimentally infected with Yersinia pestis to determine optimum sample storage conditions over time. Blood samples collected on filter paper were stored for 454 d or more in four groups: 1) at ambient temperature and at ambient relative humidity, 2) at ambient temperature with desiccant, 3) at 4 C with desiccant, and 4) at −20 C with desiccant. Samples stored at 4 C or −20 C with desiccant had detectable antibody for a longer period of time than the samples stored at room temperature.
- Subjects :
- Desiccant
Paper
Chromatography
Ecology
Filter paper
040301 veterinary sciences
Yersinia pestis
Sample (material)
030231 tropical medicine
Temperature
Animals, Wild
04 agricultural and veterinary sciences
Biology
Coyotes
Antibodies
Microbiology
Specimen Handling
0403 veterinary science
03 medical and health sciences
0302 clinical medicine
Animals
Relative humidity
Ecology, Evolution, Behavior and Systematics
Antibody detection
Subjects
Details
- ISSN :
- 19433700
- Volume :
- 52
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of wildlife diseases
- Accession number :
- edsair.doi.dedup.....9f259e7a6f4ce83d3234134cfe582af8