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Vibrational spectroscopy coupled to classical least square analysis, a new approach for determination of skin moisturizing agents' mechanisms
- Source :
- Skin Research and Technology. 20:282-292
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Background Skin dryness is an omnipresent symptom in various types of skin disorders. Thereby, a large panel of skin care products is developed for therapeutic purposes. However, there is still a lack of non-invasive methods to determine the mechanisms of action of moisturizers at the molecular level. Methods In the present study, confocal Raman spectroscopy coupled to classical least square analyses and ATR-FTIR were used to investigate the effect of different molecules on the stratum corneum (SC) hydration degree and barrier state. First, hygroscopic property was determined by analyzing samples at 90% RH; secondly, the water barrier function was evaluated after the dehydration process (4% RH). The molecules penetration kinetics across SC were also studied for 2 h. Results Using the present approach, glycerin and propylene glycol were found to be humectants; lanoline showed occlusive action, lactic acid has both humectant and barrier enhancer properties, and ethylhexyl palmitate and caprylic/capric acid triglyceride seemed to be emollients. These observations are in accordance with literature. Conclusion The present method non-invasively characterizes the mechanism of action of tested molecules. This may improve knowledge of new molecules' structure–activity relationship and help make an effective therapeutic concept dealing with the various skin dysfunctions.
- Subjects :
- Adult
Skin Absorption
Kinetics
Dermatology
In Vitro Techniques
Spectrum Analysis, Raman
Sensitivity and Specificity
Ethylhexyl palmitate
Humectant
chemistry.chemical_compound
Body Water
Stratum corneum
medicine
Humans
Organic chemistry
Computer Simulation
Diagnosis, Computer-Assisted
Least-Squares Analysis
Models, Statistical
Chromatography
Emollients
Reproducibility of Results
Penetration (firestop)
Middle Aged
Skin Care
Lactic acid
medicine.anatomical_structure
Mechanism of action
chemistry
Capric Acid
Data Interpretation, Statistical
Female
Epidermis
medicine.symptom
Algorithms
Subjects
Details
- ISSN :
- 0909752X
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Skin Research and Technology
- Accession number :
- edsair.doi.dedup.....9f2385b247d9ee186624ed2e606d4163