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Qualitative screening of veterinary anti-microbial agents in tissues, milk, and eggs of food-producing animals using liquid chromatography coupled with tandem mass spectrometry
- Source :
- Journal of Chromatography B. :82-88
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- A method for the analysis of 120 drugs in animal derived food was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). These analytes belong to 12 families of veterinary anti-microbial agents (quinolones, macrolides, β-lactams, nitroimidazoles, sulfonamides, lincomycines, chloramphenicols, quinoxalines, tetracyclines, polypeptides, and antibacterial synergists) as well as other compounds not assigned to a particular drug family. The animal derived food samples include muscle and liver of swine, bovine, sheep, and chicken, as well as hen eggs and dairy milk. The sample preparation included ultrasound-assisted extraction (UAE) with acetonitrile-water and a final clean-up with auto solid-phase extraction (SPE) on HLB cartridges. The detection and quantification of 120 anti-microbial agents was performed using LC-MS/MS in positive and negative ion mode. The chromatographic separation was performed on a C18 column using acetonitrile and 0.1% formic acid as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) of all drugs in food-producing animals were 0.5–3.0 μg/kg and 1.5–10.0 μg/kg, respectively. The developed method was successfully utilized to monitor real samples, which demonstrated that it is a simple, fast, and robust method, and could be used as a regulatory to screen for the presence of residues from veterinary anti-microbial drugs in animal-derived foods.
- Subjects :
- Veterinary Medicine
Drug
Veterinary medicine
Analyte
Formic acid
Eggs
media_common.quotation_subject
Clinical Biochemistry
Tandem mass spectrometry
Mass spectrometry
01 natural sciences
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Anti-Infective Agents
Tandem Mass Spectrometry
Animals
Sample preparation
media_common
Detection limit
Chromatography
010405 organic chemistry
Chemistry
010401 analytical chemistry
Extraction (chemistry)
Cell Biology
General Medicine
0104 chemical sciences
Milk
Chromatography, Liquid
Subjects
Details
- ISSN :
- 15700232
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....9f09857862b6fb0eaa625875019a6705