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Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase
- Source :
- EMBO Reports, Bohnsack, M T, Kos, M & Tollervey, D 2008, ' Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase ', EMBO Reports, vol. 9, no. 12, pp. 1230-1236 . https://doi.org/10.1038/embor.2008.184
- Publication Year :
- 2008
- Publisher :
- Nature Publishing Group, 2008.
-
Abstract
- In yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA—U3, U14 and snR30—whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the pre-ribosome association to all 75 yeast snoRNAs in strains depleted of eight putative helicases implicated in 40S subunit synthesis. For the modification-guide snoRNAs, we found no clear evidence for the involvement of these helicases in the association or dissociation of pre-ribosomes. However, the DEAD box helicase Rok1 was required specifically for the release of snR30. Point mutations in motif I, but not in motif III, of the helicase domain of Rok1 impaired the release of snR30, but this was less marked than in strains depleted of Rok1, and resulted in a dominant-negative growth phenotype. Dissociation of U3 and U14 from pre-ribosomes is also dependent on helicases, suggesting that release of the essential snoRNAs might differ mechanistically from release of the modification-guide snoRNAs. Keywords: ribosome biogenesis; RNA helicase; snoRNA
- Subjects :
- RNA helicase
Saccharomyces cerevisiae Proteins
Protein subunit
Saccharomyces cerevisiae
Scientific Report
Amino Acid Motifs
Ribosome biogenesis
ribosome biogenesis
snoRNA
Ribosome
Biochemistry
DEAD-box RNA Helicases
03 medical and health sciences
0302 clinical medicine
ddc:570
Genetics
RNA, Small Nucleolar
Eukaryotic Small Ribosomal Subunit
Small nucleolar RNA
Molecular Biology
030304 developmental biology
0303 health sciences
biology
urogenital system
Genetic Complementation Test
Helicase
RNA, Fungal
biology.organism_classification
RNA Helicase A
Cell biology
Molecular Weight
Mutation
biology.protein
Ribosomes
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14693178 and 1469221X
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- EMBO Reports
- Accession number :
- edsair.doi.dedup.....9f0275e737c08debfd9a0565540e6af7