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Reduced Immunohistochemical Expression of Hnf1β and FoxA2 in Liver Tissue Can Discriminate Between Biliary Atresia and Other Causes of Neonatal Cholestasis

Authors :
Noha L Ibrahim
Nermine A. Ehsan
Mohamed O Abd El-Fatah
Ghada M Naser
Mervat M Sultan
Usama F Shaalan
Source :
Applied Immunohistochemistry & Molecular Morphology. 27:e32-e38
Publication Year :
2019
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2019.

Abstract

Biliary atresia (BA) is a necroinflammatory occlusive cholangiopathy that affects infants. Genetic and environmental factors has been proposed for its occurrence. The objectives of this study was to investigate the protein expression of 2 important genes regulating ductal plate remodeling, hepatocyte nuclear factor 1-beta (Hnf1β) and the fork head box protein A2 (FoxA2) in liver tissue from patients with BA and to compare their expression with other causes of neonatal cholestasis (NC). This retrospective study included 60 pediatric patients, 30 with BA and 30 with NC. Immunohistochemistry of Hnf1β and FoxA2 was performed on liver tissues from studied patients as well as 20 healthy subjects. Statistical analysis between immunohistochemistry results and other parameters was performed. Liver tissue from patients with BA revealed reduced Hnf1β and FoxA2 immunoexpression. A strong significant statistical difference between BA and NC group (P

Details

ISSN :
15412016
Volume :
27
Database :
OpenAIRE
Journal :
Applied Immunohistochemistry & Molecular Morphology
Accession number :
edsair.doi.dedup.....9ee21812a028032b7537eb3ffae8731b
Full Text :
https://doi.org/10.1097/pai.0000000000000638