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Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
- Source :
- Nutrients, Vol 11, Iss 4, p 831 (2019), Nutrients, Volume 11, Issue 4
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-&gamma<br />and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung.
- Subjects :
- Male
0301 basic medicine
ARDS
Lipopolysaccharide
Glutathione reductase
lung mechanics
Leukocyte Count
chemistry.chemical_compound
0302 clinical medicine
Lung
chemistry.chemical_classification
reactive oxygen species
Mice, Inbred BALB C
Respiratory Distress Syndrome
Nutrition and Dietetics
medicine.diagnostic_test
Glutathione peroxidase
respiratory system
Interleukin-10
Glutathione Reductase
medicine.anatomical_structure
030220 oncology & carcinogenesis
glutamine
Female
medicine.symptom
lcsh:Nutrition. Foods and food supply
extracellular traps
medicine.medical_specialty
Inflammation
pulmonary acute respiratory distress syndrome
lcsh:TX341-641
Article
Interferon-gamma
03 medical and health sciences
Internal medicine
medicine
Animals
Peroxidase
Glutathione Peroxidase
business.industry
DNA
Pneumonia
medicine.disease
respiratory tract diseases
Endotoxins
Pulmonary Alveoli
Glutamine
Disease Models, Animal
030104 developmental biology
Bronchoalveolar lavage
Endocrinology
chemistry
Extracellular traps
Lung mechanics
Pulmonary acute respiratory distress syndrome
Reactive oxygen species
Food Science
business
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....9ed89fa506486c049edded5aad72de12