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Bacterial diversity of symptomatic primary endodontic infection by clonal analysis
Bacterial diversity of symptomatic primary endodontic infection by clonal analysis
- Source :
- Brazilian Oral Research v.30 n.1 2016, Brazilian Oral Research, Sociedade Brasileira de Pesquisa Odontológica (SBPqO), instacron:SBPQO, Repositório Institucional da UFRGS, Universidade Federal do Rio Grande do Sul (UFRGS), instacron:UFRGS, Brazilian Oral Research, Vol 30, Iss 1, Brazilian Oral Research, Volume: 30, Issue: 1, Article number: e103, Published: 10 OCT 2016
- Publication Year :
- 2016
- Publisher :
- Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2016.
-
Abstract
- The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38.15%) were taxa reported as yet-uncultivable or as yet-uncharacterized species. Prevotella spp., Fusobacterium nucleatum, Filifactor alocis, and Peptostreptococcus stomatis were the most frequently detected species, followed by Dialister invisus, Phocaeicola abscessus, the uncharacterized Lachnospiraceae oral clone, Porphyromonas spp., and Parvimonas micra. Eight phyla were detected and the most frequently identified taxa belonged to the phylum Firmicutes (43.5%), followed by Bacteroidetes (22.5%) and Proteobacteria (13.2%). No species was detected in all studied samples and some species were identified in only one case. It was concluded that acute primary endodontic infection is characterized by wide bacterial diversity and a high intersubject variability was observed. Anaerobic Gram-negative bacteria belonging to the phylum Firmicutes, followed by Bacteroidetes, were the most frequently detected microorganisms.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Periapical Abscess
030106 microbiology
Microbiologia
Porphyromonas
Polymerase Chain Reaction
Microbiology
Endodontics
03 medical and health sciences
0302 clinical medicine
RNA, Ribosomal, 16S
Prevotella
Humans
General Materials Science
Cloning, Molecular
Parvimonas micra
Phylotype
Genomic Library
Gram-Negative Anaerobic Bacteria
biology
Sequence Analysis, RNA
Microbiota
Lachnospiraceae
Dental Pulp Diseases
Molecular
Bacteroidetes
Endodontia
030206 dentistry
biology.organism_classification
lcsh:RK1-715
Bactérias
lcsh:Dentistry
Dental Pulp Cavity
Proteobacteria
Fusobacterium nucleatum
Gram-Negative Bacterial Infections
Genome, Bacterial
Cloning
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Oral Research v.30 n.1 2016, Brazilian Oral Research, Sociedade Brasileira de Pesquisa Odontológica (SBPqO), instacron:SBPQO, Repositório Institucional da UFRGS, Universidade Federal do Rio Grande do Sul (UFRGS), instacron:UFRGS, Brazilian Oral Research, Vol 30, Iss 1, Brazilian Oral Research, Volume: 30, Issue: 1, Article number: e103, Published: 10 OCT 2016
- Accession number :
- edsair.doi.dedup.....9ea39fe9e35072098bd5bdcd0c6949b6