The Inhibitory Effects of Djulis (Chenopodium formosanum) and Its Bioactive Compounds on Adipogenesis in 3T3-L1 Adipocytes

Authors :: Charng-Cherng Chyau
Chin-Chen Chu
Shih-Ying Chen
Pin-Der Duh
Source :: Molecules, Vol 23, Iss 7, p 1780 (2018), Molecules, Volume 23, Issue 7, Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Publication Year :: 2018
Publisher :: MDPI AG, 2018.
Abstract: The aim of this study was to provide new insights into the role of the ethanolic extracts of Djulis (Chenopodium formosanum, EECF) and its bioactive compounds in preventing adipogenesis in 3T3-L1 adipocytes. The results demonstrated EECF significantly inhibited oil red O-stained material (OROSM), triglyceride levels and glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 adipocytes. The expression of the critical molecules involved in lipid synthesis such as PPAR&gamma<br />C/EBP&alpha<br />and SREBP-1c was attenuated in EECF-treated cells. According to HPLC-DAD and HPLC-MS/MS analysis, rutin, kaempferol, betanin and another nine compounds were present in EECF. The suppression of lipid accumulation by rutin, kaempferol and betanin occurred by decreasing the gene expression of PPAR&gamma<br />and SREBP-1c. Taken together, these findings suggest the presence of bioactive compounds in EECF may partly account for the anti-adipogenesis of EECF and EECF is therefore a potentially lipid lowering functional food.

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