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Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model
- Source :
- Journal of biomedical materials research. Part AREFERENCES. 110(1)
- Publication Year :
- 2021
-
Abstract
- Experimental studies demonstrated antibodies against matrix and coating of polyester-based vascular prostheses. Thus, this study examined associations of these antibodies with serum cytokines (IL-2, IL-4, and IL-10) and local inflammatory reactions. Rats (n = 8/group) intramuscularly received prosthesis segments [PET-C, PET-G, and PET-A groups: polyethylene terephthalate (PET)-based prostheses coated with bovine collagen and gelatin or human serum albumin, respectively; uncoated polytetrafluoroethylene-based (PTFE) prosthesis], with sham-operated controls. Blood was drawn pre-operatively and weekly until day 22. Polymer-specific or coating-specific antibodies and cytokines were detected by enzyme immunoassays, inflammatory reactions were immunohistochemically evaluated on day 23. Polymer-specific antibodies were detected in all PET-groups using uncoated PET as antigenic target, but not for PTFE or controls, coating-specific antibodies only for PET-A. IL-10 was increased in all PET-groups and correlated with polymer-specific antibodies for PET-G and PET-A. IL-2 was increased for PET-A, but overall correlated with PET-specific antibodies. IL-4 remained unchanged in all groups. Intense local inflammatory reactions (ED1+ /ED2+ macrophages and T lymphocytes) were found within all PET-groups, but only minor for PTFE or controls. In conclusion, PET-specific antibodies were associated with increased IL-10 and along with concurrent coating-specific antibodies also with increased IL-2, indicating a specific T cell response. Thus, matrix and/or coating of polymeric vascular prostheses elicit distinct systemic immune reactions, probably influencing local inflammatory reactions.
- Subjects :
- medicine.medical_specialty
Materials science
food.ingredient
medicine.medical_treatment
Biomedical Engineering
Matrix (biology)
Prosthesis
Gelatin
Biomaterials
chemistry.chemical_compound
food
Antigen
Internal medicine
medicine
Polyethylene terephthalate
Animals
Polytetrafluoroethylene
biology
Polyethylene Terephthalates
Metals and Alloys
Human serum albumin
Blood Vessel Prosthesis
Rats
Polyester
Disease Models, Animal
Endocrinology
chemistry
Antibody Formation
Ceramics and Composites
biology.protein
Cytokines
Cattle
Antibody
medicine.drug
Subjects
Details
- ISSN :
- 15524965
- Volume :
- 110
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical materials research. Part AREFERENCES
- Accession number :
- edsair.doi.dedup.....9e8242c67cc4ed73b521cffd7bf4b2d3