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Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution
- Source :
- International Journal of Biological Macromolecules. 175:79-86
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Metal organic frameworks (MOFs) are hybrid organic inorganic materials with unique properties such as well-defined pore structure, extremely high surface area, excellent chemical-thermal stability. MOFs-based constructs have been extensively engineered and used for applications, such as enzyme immobilization for bio-catalysis. To obtained a zeolitic imidazole framework-8 (ZIF-8) for enzyme immobilization, Candida rugosa lipase (CRL) was pretreated with calix [4]arene tetracarboxylic acid (Calix) and reacted with Zn and imidazole by co-precipitation method. The prepared biocomposite was characterized by SEM, EDX, FT-IR, and XRD. The prepared CRL@Calix-ZIF-8 with high encapsulation efficiency showed improved resistance to alkali and thermal conditions. The CRL@Calix-ZIF-8 with the biocatalytic activity was 2-folds higher than that of the CRL@ZIF-8 (without Calix). The free lipase lost its catalytic activity completely at 60 °C after 100 min, while the CRL@Calix-ZIF-8 and CRL@ZIF-8 retained about 84% and 73%. It was found that CRL@Calix-ZIF-8 and CRL@ZIF-8 still retained ~83 and 67% of catalytic activity after its 6th use, respectively. The kinetic resolution of the immobilized lipases was examined for enantioselective hydrolysis of racemic naproxen methyl ester. CRL@Calix-ZIF-8 showed enantioselectivity against the racemic naproxen methyl ester, with E = 183 and 131 compared to the CRL@ZIF-8.
- Subjects :
- Immobilized enzyme
Carboxylic Acids
02 engineering and technology
Biochemistry
Catalysis
Kinetic resolution
03 medical and health sciences
chemistry.chemical_compound
Hydrolysis
Phenols
Structural Biology
Enzyme Stability
Spectroscopy, Fourier Transform Infrared
Imidazole
Lipase
Molecular Biology
Metal-Organic Frameworks
Candida
030304 developmental biology
0303 health sciences
biology
Temperature
Esters
Stereoisomerism
General Medicine
Hydrogen-Ion Concentration
Enzymes, Immobilized
021001 nanoscience & nanotechnology
Enzymes
Kinetics
chemistry
Biocatalysis
biology.protein
Metal-organic framework
Calixarenes
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 175
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....9e7c1bb9a0aaecd06fd2921a4f1ea785