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PAQR3 gene expression and its methylation level in colorectal cancer tissues
- Source :
- Oncology Letters
- Publication Year :
- 2016
- Publisher :
- D.A. Spandidos, 2016.
-
Abstract
- The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Colorectal cancer
mRNA
colorectal cancer
Biology
PAQR3
03 medical and health sciences
0302 clinical medicine
Western blot
Gene expression
medicine
Oncogene
medicine.diagnostic_test
Methylation
Articles
Cell cycle
medicine.disease
Molecular medicine
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
western blot analysis
methylation
Infiltration (medical)
Subjects
Details
- Language :
- English
- ISSN :
- 17921082 and 17921074
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....9e7863692664de498ed5b01cc0cdf8ec