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Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
- Source :
- Saudi Journal of Biological Sciences, Saudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6853-6860 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- The Asian honey bee species i.e., Apis cerana (the eastern honey bee), A. dorsata (the giant honey bee), and the western or European honey bee (A. mellifera) collected from Pakistan were studied using partial sequences from two mitochondrial genes (i) the Cytochrome c oxidase I (COI) and (ii) the mitochondrially encoded NADH dehydrogenase 5 (ND5) and then compared with other honey bees sequences (already submitted from different countries around the globe) obtained after the national center for biotechnology information (NCBI). DNA sequences were analyzed employing molecular evolutionary genetics analysis and Kimura 2-parameter model, neighbor-joining method was applied to investigate phylogenetic relationships, and DNA sequence polymorphism was applied to measure the genetic diversity within the genus Apis. The phylogenetic analyses yielded consistent results. Based on COI gene fragment in two Asian and European honey bee species from Pakistan and from other countries showed considerable genetic diversity levels and deviation among the species. While in contrast the phylogenetic analyses based on ND5 gene fragment in Asian and European honey bee species from Pakistan and other countries showed comparatively higher genetic diversity indices and variations than the COI gene. So, in the genus Apis, the mitochondrial ND5 region has shown the possibility to answer the interactions among species. A further detailed work (by linking the analysis of other genomic and mitochondrial genes) is required for good quality solution to establish the concise genetic diversity and interaction among the Apis species. The objective of this study was to explore the extent of genetic differences and phylogenetic links among the three kinds of honey bee species from Pakistan and comparing them with other bee species around the globe.
- Subjects :
- Genetic diversity
Mitochondrial DNA
Phylogenetic tree
Human evolutionary genetics
QH301-705.5
A. mellifera
Molecular analysis
fungi
DNA markers
Genetic analysis
food and beverages
Honey bee
Biology
biology.organism_classification
Classification
DNA sequencing
A. dorsata
Evolutionary biology
behavior and behavior mechanisms
Original Article
Biology (General)
General Agricultural and Biological Sciences
Gene
Apis cerana
Subjects
Details
- Language :
- English
- ISSN :
- 22137106 and 1319562X
- Volume :
- 28
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....9e311fc4104477823a7672d7ad8e3c45