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Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts
- Source :
- Molecules, Molecules, Vol 18, Iss 2, Pp 2200-2211 (2013), Molecules; Volume 18; Issue 2; Pages: 2200-2211
- Publication Year :
- 2012
-
Abstract
- The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.
- Subjects :
- Male
GPX1
Antioxidant
medicine.medical_treatment
SOD2
Pharmaceutical Science
Radiation-Protective Agents
Antioxidants
Gene Expression Regulation, Enzymologic
Article
Analytical Chemistry
Superoxide dismutase
lcsh:QD241-441
Gelam honey
lcsh:Organic chemistry
antioxidant enzymes
Drug Discovery
Gene expression
medicine
Humans
Physical and Theoretical Chemistry
Child
human diploid fibroblasts
chemistry.chemical_classification
Glutathione Peroxidase
biology
Superoxide Dismutase
Glutathione peroxidase
Organic Chemistry
food and beverages
Honey
gamma-irradiation damage
Fibroblasts
Catalase
Molecular biology
Diploidy
Enzyme assay
Biochemistry
chemistry
Chemistry (miscellaneous)
Gamma Rays
biology.protein
Molecular Medicine
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 18
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....9e2959db9575d1325ec839695fe8ceaa