Back to Search
Start Over
DNA methylation profiling as a tool for testicular germ cell tumors subtyping
- Source :
- Epigenomics. 10:1511-1523
- Publication Year :
- 2018
- Publisher :
- Future Medicine Ltd, 2018.
-
Abstract
- Aim: Assess differential patterns of selected five genes’ promoter methylation among testicular germ cell tumors (TGCT) subtypes. Materials & methods: CRIPTO, HOXA9, MGMT, RASSF1A and SCGB3A1 promoter methylation levels were evaluated by quantitative methylation-specific PCR in 161 TGCT and 16 controls. Associations between clinicopathological parameters and promoter methylation levels were assessed, and receiver operating characteristics curve analysis was performed. Results: Promoter methylation of CRIPTO/HOXA9/SCGB3A1 panel and RASSF1A best discriminated between controls and nonseminomatous tumors or seminomas, respectively, whereas HOXA9/RASSF1A panel displayed the best discriminative performance between nonseminomatous tumor and seminomas. Significant differences in CRIPTO, MGMT and RASSF1A methylation levels were depicted between pure forms and matched mixed components of seminomas and embryonal carcinoma. HOXA9, RASSF1A and SCGB3A1 promoter methylation significantly associated with tumor stage. Conclusion: Different combinations of five genes’ promoter methylation levels discriminate among TGCT subtypes. Methylation patterns may also assist in identification of more clinically aggressive tumors.
- Subjects :
- Adult
Male
0301 basic medicine
endocrine system
Cancer Research
Adolescent
Biology
GPI-Linked Proteins
Cripto
Embryonal carcinoma
Young Adult
03 medical and health sciences
0302 clinical medicine
Testicular Neoplasms
Tumor stage
Genetics
medicine
Humans
Promoter Regions, Genetic
DNA Modification Methylases
Gene
Homeodomain Proteins
Portugal
Tumor Suppressor Proteins
Methylation
DNA Methylation
Middle Aged
Neoplasms, Germ Cell and Embryonal
Prognosis
medicine.disease
Testicular germ cell
Subtyping
Neoplasm Proteins
Dna methylation profiling
DNA Repair Enzymes
030104 developmental biology
ROC Curve
030220 oncology & carcinogenesis
Cancer research
Cytokines
Intercellular Signaling Peptides and Proteins
Subjects
Details
- ISSN :
- 1750192X and 17501911
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Epigenomics
- Accession number :
- edsair.doi.dedup.....9dc81044a6d71b61b884d21d0de18103