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Association between possession of ExoU and antibiotic resistance in pseudomonas aeruginosa
- Source :
- PLoS ONE, Vol 13, Iss 9, p e0204936 (2018), PLoS ONE
- Publication Year :
- 2018
-
Abstract
- © 2018 Subedi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Virulent strains of Pseudomonas aeruginosa are often associated with an acquired cytotoxic protein, exoenzyme U (ExoU) that rapidly destroys the cell membranes of host cells by its phospholipase activity. Strains possessing the exoU gene are predominant in eye infections and are more resistant to antibiotics. Thus, it is essential to understand treatment options for these strains. Here, we have investigated the resistance profiles and genes associated with resistance for fluoroquinolone and beta-lactams. A total of 22 strains of P. aeruginosa from anterior eye infections, microbial keratitis (MK), and the lungs of cystic fibrosis (CF) patients were used. Based on whole genome sequencing, the prevalence of the exoU gene was 61.5% in MK isolates whereas none of the CF isolates possessed this gene. Overall, higher antibiotic resistance was observed in the isolates possessing exoU. Of the exoU strains, all except one were resistant to fluoroquinolones, 100% were resistant to beta-lactams. 75% had mutations in quinolone resistance determining regions (T81I gyrA and/or S87L parC) which correlated with fluoroquinolone resistance. In addition, exoU strains had mutations at K76Q, A110T, and V126E in ampC, Q155I and V356I in ampR and E114A, G283E, and M288R in mexR genes that are associated with higher beta-lactamase and efflux pump activities. In contrast, such mutations were not observed in the strains lacking exoU. The expression of the ampC gene increased by up to nine-fold in all eight exoU strains and the ampR was upregulated in seven exoU strains compared to PAO1. The expression of mexR gene was 1.4 to 3.6 fold lower in 75% of exoU strains. This study highlights the association between virulence traits and antibiotic resistance in pathogenic P. aeruginosa.
- Subjects :
- 0301 basic medicine
Cystic Fibrosis
Pulmonology
Antibiotics
Gene Expression
lcsh:Medicine
Drug resistance
medicine.disease_cause
Pathology and Laboratory Medicine
Eye Infections, Bacterial
Medicine and Health Sciences
lcsh:Science
Multidisciplinary
Antimicrobials
Pseudomonas Aeruginosa
Drugs
High-Throughput Nucleotide Sequencing
Bacterial Pathogens
Science::Biological sciences [DRNTU]
Mutant Strains
Medical Microbiology
Genetic Diseases
Pseudomonas aeruginosa
Efflux
Pathogens
Research Article
medicine.drug_class
General Science & Technology
030106 microbiology
Mutation, Missense
Virulence
Biology
Microbiology
beta-Lactamases
03 medical and health sciences
Antibiotic resistance
Autosomal Recessive Diseases
Bacterial Proteins
Gene Types
Microbial Control
Pseudomonas
Drug Resistance, Bacterial
medicine
Genetics
Point Mutation
Humans
Pseudomonas Infections
Gene
Microbial Pathogens
Pharmacology
Clinical Genetics
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Eye infection
Fibrosis
Repressor Proteins
Amino Acid Substitution
Antibiotic Resistance
Mutation
Regulator Genes
lcsh:Q
Antimicrobial Resistance
Virulence Traits
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Vol 13, Iss 9, p e0204936 (2018), PLoS ONE
- Accession number :
- edsair.doi.dedup.....9d88202426b1b95661ed72ec198790c7