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Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy: An endemic plant from Turkey
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 171:118-125
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- This study was geared towards assessing the possible antioxidant, enzyme inhibitory, and cytotoxic activities of ethyl acetate, methanol, and water extracts of Sideritis ozturkii Aytac & Aksoy. The phytochemical profiles of the studied extracts were characterised by HPLC-MS/MS. The methanol extract, rich in phenolics (78.04 mg gallic acid equivalent/g), exhibited the strongest antioxidant activities. However, the ethyl acetate extract was the most active extract in the enzyme inhibitory assays. The water extract of S. ozturkii (1 mg/ml, 48 h incubation) slightly inhibited (22%) growth of human breast cancer cell line (MDA-MB-231 cells). On the other hand, the ethyl acetate and methanol extracts showed strong inhibition (98% and 97%, respectively) of MDA-MB-231 cells and caused apoptotic cell death. Scientific data generated from this study further appraises the multiple biological activities of plants belonging to the Sideritis genus. In addition, preliminary evidence gathered from the current investigation advocates for further studies geared towards the preparation of therapeutic formulations from S. ozturkii.
- Subjects :
- Antioxidant
Turkey
Cell Survival
medicine.medical_treatment
Clinical Biochemistry
Cell
Ethyl acetate
Pharmaceutical Science
Acetates
01 natural sciences
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
Cell Line, Tumor
Drug Discovery
medicine
Humans
Gallic acid
Enzyme Inhibitors
Incubation
Spectroscopy
biology
Traditional medicine
Plant Extracts
010405 organic chemistry
Methanol
010401 analytical chemistry
Water
biology.organism_classification
Antineoplastic Agents, Phytogenic
0104 chemical sciences
medicine.anatomical_structure
Phytochemical
chemistry
Solvents
Sideritis
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 171
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....9d6d530c3d38bce31baa77cc8458fd20