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Immunoglobulin G antibodies to merozoite surface antigens are associated with recovery from chloroquine-resistant Plasmodium falciparum in Gambian children
- Source :
- Infection and Immunity, 74, 5, pp. 2887-93, Infection and Immunity, 74, 2887-93
- Publication Year :
- 2006
-
Abstract
- We examined the hypothesis that recovery from uncomplicated malaria in patients carrying drug-resistantPlasmodium falciparumis a measure of acquired functional immunity and may therefore be associated with humoral responses to candidate vaccine antigens. Gambian children with malaria were treated with chloroquine in 28-day trials, and recovery was defined primarily as the absence of severe clinical malaria at any time and absence of parasitemia with fever after 3 days. Plasma samples from these children were assayed by enzyme-linked immunosorbent assay for immunoglobulin G (IgG) to recombinant merozoite antigens: apical membrane antigen 1 (AMA-1) and the 19-kDa C-terminal region of merozoite surface protein 1 (MSP-119), including antigenic variants of MSP-119with double and triple substitutions. Antigen-specific IgG was more frequent in children who recovered, particularly that for MSP-119(age-adjusted odds ratios: 0.32 [95% confidence interval, 0.05, 1.87;P= 0.168] for AMA-1, 0.19 [0.03, 1.11;P= 0.019] for recombinant MSP-119, 0.24 [0.04, 1.31;P= 0.032] for the recombinant MSP-119double variant, and 0.18 [0.03, 0.97;P= 0.013] for the triple variant). IgG titers to MSP-119and to the triple variant were higher in plasma samples taken 7 days after chloroquine treatment from children who carried resistant parasites but recovered and remained parasite free. Moreover, in children who were parasitemic on day 14 or day 28, there was an age-independent relationship between parasite density and IgG to both MSP-119and the triple variant (coefficients of −0.550 and −0.590 andPvalues of 0.002 and 0.001, respectively). The results validate the use of this approach to identify antigens that are associated with protection from malaria.
- Subjects :
- 030231 tropical medicine
Immunology
Plasmodium falciparum
Protozoan Proteins
Antibodies, Protozoan
Antigens, Protozoan
Parasitemia
Microbiology
Immunoglobulin G
03 medical and health sciences
Antimalarials
0302 clinical medicine
Antigen
Chloroquine
parasitic diseases
Malaria Vaccines
medicine
Animals
Humans
Apical membrane antigen 1
Malaria, Falciparum
Child
Merozoite Surface Protein 1
Retrospective Studies
0303 health sciences
biology
030306 microbiology
Poverty-related infectious diseases [N4i 3]
Infant
Membrane Proteins
medicine.disease
biology.organism_classification
Virology
3. Good health
Infectious Diseases
Child, Preschool
Microbial Immunity and Vaccines
biology.protein
Parasitology
Microbial pathogenesis and host defense [UMCN 4.1]
Antibody
Malaria
medicine.drug
Immunity, infection and tissue repair [NCMLS 1]
Subjects
Details
- ISSN :
- 00199567
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity, 74, 5, pp. 2887-93, Infection and Immunity, 74, 2887-93
- Accession number :
- edsair.doi.dedup.....9d5181bad807a12752550c55d0e96cd9