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Characterization of a High Hierarchical Regulator, PtrGATA12, Functioning in Differentially Regulating Secondary Wall Component Biosynthesis in Populus trichocarpa
- Source :
- Frontiers in Plant Science, Vol 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- In plants, GATA transcription factors (TFs) have been reported to play vital roles in to a wide range of biological processes. To date, there is still no report about the involvement and functions of woody plant GATA TFs in wood formation. In this study, we described the functional characterization of a Populus trichocarpa GATA TF, PtrGATA12, which encodes a nuclear-localized transcriptional activator predominantly expressing in developing xylem tissues. Overexpression of PtrGATA12 not only inhibited growths of most phenotypic traits and biomass accumulation, but also altered the expressions of some master TFs and pathway genes involved in secondary cell wall (SCW) and programmed cell death, leading to alternated SCW components and breaking forces of stems of transgenic lines. The significant changes occurred in the contents of hemicellulose and lignin and SCW thicknesses of fiber and vessel that increased by 13.5 and 10.8%, and 20.83 and 11.83%, respectively. Furthermore, PtrGATA12 bound directly to the promoters of a battery of TFs and pathway genes and activated them; the binding sites include two cis-acting elements that were specifically enriched in their promoter regions. Taken together, our results suggest PtrGATA12, as a higher hierarchical TF on the top of PtrWND6A, PtrWND6B, PtrMYB152, and PtrMYB21, exert a coordinated regulation of SCW components biosynthesis pathways through directly and indirectly controlling master TFs, middle-level TFs, and further downstream pathway genes of the currently known hierarchical transcription network that governs SCW formation.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Populus trichocarpa
PtrGATA12
Regulator
coordinated regulation
Plant Science
Biology
01 natural sciences
SB1-1110
03 medical and health sciences
Transcription (biology)
Binding site
Gene
hierarchical regulator
fungi
Plant culture
Promoter
biology.organism_classification
Cell biology
030104 developmental biology
biological characterization
GATA transcription factor
secondary cell wall
Secondary cell wall
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1664462X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....9d3bb90fda72905db32ceb1cfe56ce1c