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Plant Pellets: A Compatible Vegan Feedstock for Preparation of Plant-Based Culture Media and Production of Value-Added Biomass of Rhizobia

Authors :
Mennatullah Abdou
Mohamed S. Sarhan
Hanan A. Goda
Hassan-Sibroe A. Daanaa
Reem Hamed
Hanan H. Youssef
Mohamed Fayez
Mervat A. Hamza
Mahmoud El-Tahan
Mohamed T. Abbas
Silke Ruppel
Nabil A. Hegazi
Source :
Sustainability, Volume 12, Issue 20, Sustainability, Vol 12, Iss 8389, p 8389 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Although plant-based culture media enhances in vitro cultivation of rhizobacteria, studies assessing their biomass potential for large-scale applications are lacking. Here, we advance plant pellets (PPs) as a novel technology to unlock the potential of such vegan culture media for biomass production of Rhizobium leguminosarum. PP formulations were based on mixtures of Egyptian clover powder and the agro-byproducts glycerol and molasses. These mixtures were either contained or not contained in teabags during culture media preparation. Metrics of biomass included colony forming units, optical density (OD600nm), and cell dry weight (DW). Biomass comparisons between culture media based on PPs and standard yeast extract mannitol (YEM) revealed that the following PPs composition, contained in teabags, cultivated rhizobia at levels comparable to YEM: 16 g clover powder, 5% molasses, and 0.8% glycerol. This PPs composition enabled shorter generation times of rhizobia (PP: 3.83 h, YEM: 4.28 h). Strikingly, PPs mixtures supplemented with 10% molasses and not contained in teabags promoted rhizobia without apparent lag phases and produced 25% greater DW than YEM. PPs potentiate the use of dehydrated vegan feedstocks for both plant microbiota cultivation and biomass production and appear as cost- and labor-effective tools, easy to handle and store for plant-based culture media preparation.

Details

ISSN :
20711050
Volume :
12
Database :
OpenAIRE
Journal :
Sustainability
Accession number :
edsair.doi.dedup.....9d232e02503385d139dcc3ba86d0fcf4