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Plant Pellets: A Compatible Vegan Feedstock for Preparation of Plant-Based Culture Media and Production of Value-Added Biomass of Rhizobia
- Source :
- Sustainability, Volume 12, Issue 20, Sustainability, Vol 12, Iss 8389, p 8389 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Although plant-based culture media enhances in vitro cultivation of rhizobacteria, studies assessing their biomass potential for large-scale applications are lacking. Here, we advance plant pellets (PPs) as a novel technology to unlock the potential of such vegan culture media for biomass production of Rhizobium leguminosarum. PP formulations were based on mixtures of Egyptian clover powder and the agro-byproducts glycerol and molasses. These mixtures were either contained or not contained in teabags during culture media preparation. Metrics of biomass included colony forming units, optical density (OD600nm), and cell dry weight (DW). Biomass comparisons between culture media based on PPs and standard yeast extract mannitol (YEM) revealed that the following PPs composition, contained in teabags, cultivated rhizobia at levels comparable to YEM: 16 g clover powder, 5% molasses, and 0.8% glycerol. This PPs composition enabled shorter generation times of rhizobia (PP: 3.83 h, YEM: 4.28 h). Strikingly, PPs mixtures supplemented with 10% molasses and not contained in teabags promoted rhizobia without apparent lag phases and produced 25% greater DW than YEM. PPs potentiate the use of dehydrated vegan feedstocks for both plant microbiota cultivation and biomass production and appear as cost- and labor-effective tools, easy to handle and store for plant-based culture media preparation.
- Subjects :
- 0106 biological sciences
lcsh:TJ807-830
Geography, Planning and Development
lcsh:Renewable energy sources
Biomass
Management, Monitoring, Policy and Law
Raw material
medicine.disease_cause
Rhizobacteria
01 natural sciences
Rhizobium leguminosarum
Rhizobia
03 medical and health sciences
chemistry.chemical_compound
biomass production
medicine
Glycerol
Yeast extract
Food science
lcsh:Environmental sciences
plant-based culture media
030304 developmental biology
lcsh:GE1-350
0303 health sciences
biology
Renewable Energy, Sustainability and the Environment
lcsh:Environmental effects of industries and plants
plant pellets
biology.organism_classification
sustainable agriculture
lcsh:TD194-195
chemistry
Composition (visual arts)
feedstocks for rhizobacteria
010606 plant biology & botany
Subjects
Details
- ISSN :
- 20711050
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Sustainability
- Accession number :
- edsair.doi.dedup.....9d232e02503385d139dcc3ba86d0fcf4