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A Preliminary Linkage Map of the Tick, Ixodes scapularis
- Source :
- Experimental and Applied Acarology. 28:107-126
- Publication Year :
- 2002
- Publisher :
- Springer Science and Business Media LLC, 2002.
-
Abstract
- A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximately 10(9) bp, the relationship of physical to genetic distance is approximately 300 kb/cM in the I. scapularis genome.
- Subjects :
- Male
Genetics
DNA, Complementary
Genome
Ixodes
Ecology
Genetic Linkage
Chromosome Mapping
General Medicine
Biology
Random Amplified Polymorphic DNA Technique
RAPD
Genetic distance
Animal ecology
Genetic linkage
Ixodes scapularis
Insect Science
Animals
Microsatellite
Female
Genome size
Crosses, Genetic
Microsatellite Repeats
Sequence Tagged Sites
Subjects
Details
- ISSN :
- 01688162
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Experimental and Applied Acarology
- Accession number :
- edsair.doi.dedup.....9cf091625e88a38b7ad4bda0d668232b