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Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks
- Source :
- Microbial Pathogenesis. :6-11
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- To construct a novel live, attenuated Salmonella vaccine, the lon, cpxR and cpdB genes were deleted from a wild-type Salmonella enterica serovar Enteritidis-6 (SM-6) strain using the phage λ Red homologous recombination system, resulting in SM-△CpxR, SM-△C/Lon and SM-△C/L/CpdB. The growth curves of strain SM-△C/Lon grew more rapidly than the other strains and had OD 600 values higher than the other strains starting at the 4 h time point. The growth curves of strain SM-△C/L/CpdB were relatively flat. The colonization time of SM-△C/L/CpdB is about 8-10 days. Deleting the lon/cpxR/cpdB (SM-6) genes resulted in an approximate 10(3)-fold attenuation in virulence assessed by the analysis of the LD50 of specific pathogen-free (SPF) chicks. This result indicated that the deletion of the lon, cpxR and cpdB genes induced significant virulence attenuation. The protective effects of SM-△C/L/CpdB vaccination in SPF chicks against 5 × 10(9) colony forming units (CFU) of S. Enteritidis were resulted from the induction of an effective immune response. These findings demonstrate the potential of mutant SM-△C/L/CpdB to be used as an effective vaccine.
- Subjects :
- Serotype
Salmonella Vaccines
Virulence Factors
Salmonella enteritidis
Mutant
Virulence
Vaccines, Attenuated
Microbiology
Lethal Dose 50
Bacterial Proteins
Animals
Recombination, Genetic
Colony-forming unit
Salmonella Infections, Animal
biology
Strain (chemistry)
Immunogenicity
biology.organism_classification
Infectious Diseases
Animals, Newborn
Salmonella enterica
bacteria
Chickens
Gene Deletion
Subjects
Details
- ISSN :
- 08824010
- Database :
- OpenAIRE
- Journal :
- Microbial Pathogenesis
- Accession number :
- edsair.doi.dedup.....9ceb9ac65f4497a85a31be37c915fd0e