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Effects of catechin-rich green tea on gene expression of gluconeogenic enzymes in rat hepatoma H4IIE cells
- Source :
- Biomedical research (Tokyo, Japan). 31(3)
- Publication Year :
- 2010
-
Abstract
- Rat hepatoma H4IIE cells were stimulated with dexamethasone and dibutyryl cAMP to increase gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK). Inclusion of catechin-rich green tea beverage (GTB) in the culture medium reduced the up-regulation of these genes as well as that of hepatocyte nuclear factor 4 alpha (HNF4alpha) gene. GTB was fractionated into chloroform-soluble (Fraction I), ethyl acetatesoluble (Fraction II), methanol-soluble (Fraction III) and residual (Fraction IV) fractions. Fractions II and III containing catechins caused an attenuation of the up-regulated expression of these genes as well as the down-regulation of HNF4alpha gene expression. Fraction IV had a synergistic effect on the up-regulation by dexamethasone/dibutyryl cAMP of the PEPCK gene expression and upregulated HNF4alpha gene expression. These results suggest that GTB down-regulated the expression of the HNF4alpha gene to cause the down-regulated gene expression of gluconeogenic enzymes. One reason why GTB did not down-regulate hepatic PEPCK gene expression in previous animal experiments may be that the component(s) acting to up-regulate PEPCK gene expression was more effective in vivo than in cultured cells.
- Subjects :
- endocrine system
Biology
digestive system
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
Catechin
chemistry.chemical_compound
Liver Neoplasms, Experimental
Downregulation and upregulation
In vivo
Cell Line, Tumor
Gene expression
medicine
Animals
Insulin
Gene
Dexamethasone
DNA Primers
Base Sequence
Tea
Gluconeogenesis
General Medicine
Molecular biology
Rats
Biochemistry
chemistry
Gene Expression Regulation
Hepatocyte nuclear factor 4 alpha
Glucose-6-Phosphatase
Phosphoenolpyruvate carboxykinase
Phosphoenolpyruvate Carboxykinase (ATP)
medicine.drug
Subjects
Details
- ISSN :
- 1880313X
- Volume :
- 31
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedical research (Tokyo, Japan)
- Accession number :
- edsair.doi.dedup.....9cbf3b3d09db63467eb94c3485a58cc6