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SERPINA1 Peptides in Urine as A Potential Marker of Preeclampsia Severity
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 3, International Journal of Molecular Sciences, Vol 21, Iss 3, p 914 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Preeclampsia (PE) is a multisystem disorder associated with pregnancy and its frequency varies from 5 to 20 percent of pregnancies. Although a number of preeclampsia studies have been carried out, there is no consensus about disease etiology and pathogenesis so far. Peptides of SERPINA1 (&alpha<br />1-antitrypsin) in urine remain one of the most promising peptide markers of PE. In this study the diagnostic potential of urinary &alpha<br />1-antitrypsin peptides in PE was evaluated. The urinary peptidome composition of 79 pregnant women with preeclampsia (PE), chronic arterial hypertension (CAH), and a control group was investigated. Mann&ndash<br />Whitney U-test (p &lt<br />0.05) revealed seven PE specific SERPINA1 peptides demonstrating 52% sensitivity and 100% specificity. SERPINA1 in urine has been associated with the most severe forms of preeclampsia (p = 0.014), in terms of systolic hypertension (p = 0.01) and proteinuria (p = 0.006). According to Spearman correlation analysis, the normalized intensity of SERPINA1 urinary peptides has a similar diagnostic pattern with known diagnostic PE markers, such as sFLT/PLGF. SERPINA1 peptides were not urinary excreted in superimposed PE (PE with CAH), which is a milder form of PE. An increase in expression of SERPINA1 in the structural elements of the placenta during preeclampsia reflects a protective mechanism against hypoxia. Increased synthesis of SERPINA1 in the trophoblast leads to protein accumulation in fibrinoid deposits. It may block syncytial knots and placenta villi, decreasing trophoblast invasion. Excretion of PE specific SERPINA1 peptides is associated with syncytiotrophoblast membrane destruction degradation and increased SERPINA1 staining. It confirms that the placenta could be the origin of SERPINA1 peptides in urine. Significant correlation (p &lt<br />0.05) of SERPINA1 expression in syncytiotrophoblast membrane and cytoplasm with the main clinical parameters of severe PE proves the role of SERPINA1 in PE pathogenesis. Estimation of SERPINA1 peptides in urine can be used as a diagnostic test of the severity of the condition to determine further treatment, particularly the need for urgent surgical delivery.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Urinary system
syncytiotrophoblast
Urine
030204 cardiovascular system & hematology
Catalysis
Article
Preeclampsia
lcsh:Chemistry
Inorganic Chemistry
Pathogenesis
preeclampsia
03 medical and health sciences
0302 clinical medicine
Syncytiotrophoblast
proteomics
Internal medicine
Placenta
medicine
diagnostics
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
reproductive and urinary physiology
mass spectrometry
Proteinuria
business.industry
Organic Chemistry
Trophoblast
General Medicine
medicine.disease
female genital diseases and pregnancy complications
Computer Science Applications
030104 developmental biology
medicine.anatomical_structure
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
embryonic structures
alpha-1 antitrypsin
medicine.symptom
business
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....9cbaec17b182e199b5ce27d814ce5745