Back to Search
Start Over
Assessment of the clinical relevance of 17q25.3 copy number and three-dimensional telomere organization in non-small lung cancer patients
- Source :
- Journal of Cancer Research and Clinical Oncology. 142:749-756
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- To identify potential biomarkers that may provide new therapeutic targets or prognostic indicators for non-small cell lung cancer (NSCLC), we investigated the three-dimensional (3D) organization of telomeres and cytoband 17q25.3 copy number in NSCLC tissues. NSCLC paraffin-embedded tissue specimens from 18 patients were assessed for 3D telomere organization by 3D nuclear telomere imaging followed by quantitative analysis. Patients were stratified by smoking, histology, and EGFR status. Cytoband 17q25.3 was examined by fluorescent in situ hybridization. Data from comparative genomic hybridization and/or single nucleotide polymorphism arrays for cytoband 17q25.3 were obtained and correlated with Q-FISH and 3D telomere results. 3D telomeric profiling demonstrated that the smokers, EGFR-negative, and squamous cell carcinoma subgroups tended to have higher numbers of lower-intensity telomeres, indicative of shorter telomeres, as well as higher numbers of telomeric aggregations compared to non-smokers, EGFR-positive, and adenocarcinomas, respectively. Gains of cytoband 17q25.3 in conjunction with an increase in the control region 17p11.2 were observed in 7 of 18 (38.9 %) patients, reflecting a gain of chromosome 17. Clonal gains of cytoband 17q25.3 were observed in 11 of 18 (61 %) patients, highlighting a potential biological significance for the genes in this region in NSCLC tumourigenesis. The 3D telomere profiles may differentiate NSCLC patients with different histologies, EGFR, and smoking statuses, rendering them a potential biomarker for distinguishing these clinically relevant histological and molecular subtypes of lung cancer. Highly frequent clonal gain of cytoband 17q25.3 was also demonstrated, suggesting an important biological role for the genes in this region.
- Subjects :
- Adult
Male
0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
Lung Neoplasms
DNA Copy Number Variations
Single-nucleotide polymorphism
Adenocarcinoma
Biology
Polymorphism, Single Nucleotide
Telomere organization
03 medical and health sciences
0302 clinical medicine
Carcinoma, Non-Small-Cell Lung
Internal medicine
medicine
Humans
Lung cancer
In Situ Hybridization, Fluorescence
Survival analysis
Aged
Neoplasm Staging
Aged, 80 and over
Comparative Genomic Hybridization
Smoking
General Medicine
Middle Aged
Telomere
medicine.disease
Survival Analysis
ErbB Receptors
Chromosome 17 (human)
030104 developmental biology
030220 oncology & carcinogenesis
Female
Chromosomes, Human, Pair 17
Comparative genomic hybridization
Subjects
Details
- ISSN :
- 14321335 and 01715216
- Volume :
- 142
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer Research and Clinical Oncology
- Accession number :
- edsair.doi.dedup.....9cb42c9f296a7214680e54fef525f0d6