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CTCF deficiency causes expansion of the sensory domain in the mouse cochlea
- Source :
- Biochemical and Biophysical Research Communications. 512:896-901
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The cochlea in the mammalian inner ear is a sensitive and sharply organized sound-detecting structure. The proper specification of neurosensory-competent domain in the otic epithelium is required for the formation of mature neuronal and sensory domains. Genetic studies have provided many insights into inner ear development, but there have been few epigenetic studies of inner ear development. CTCF is an epigenetic factor that plays a pivotal role in the organization of global chromatin conformation. To determine the role of CTCF in the otic sensory formation, we made a conditional knockout of Ctcf in the developing otic epithelium by crossing Ctcffl/fl mice with Pax2-Cre mice. Ctcf deficiency resulted in extra rows of auditory hair cells in the shortened cochlea on mouse embryonic day 14.5 (E14.5) and E17.5. The massive and ectopic expression of sensory specifiers such as Jag1 and Sox2 indicated that the sensory domain was expanded in the Ctcf-deficient cochlea. Other regulators of the sensory domain such as Bmp4, Gata3, and Fgf10 were not affected. These results suggest that CTCF plays a role in the regulation of the sensory domain in mammalian cochlear development.
- Subjects :
- 0301 basic medicine
CCCTC-Binding Factor
Biophysics
Sensory system
Bone Morphogenetic Protein 4
GATA3 Transcription Factor
Biology
Biochemistry
03 medical and health sciences
0302 clinical medicine
SOX2
Hair Cells, Auditory
Conditional gene knockout
otorhinolaryngologic diseases
medicine
Animals
Inner ear
Molecular Biology
Cochlea
Mice, Knockout
Hair cell differentiation
SOXB1 Transcription Factors
PAX2 Transcription Factor
Gene Expression Regulation, Developmental
Cell Differentiation
Cell Biology
Cell biology
030104 developmental biology
medicine.anatomical_structure
CTCF
030220 oncology & carcinogenesis
embryonic structures
Ectopic expression
sense organs
Fibroblast Growth Factor 10
Jagged-1 Protein
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 512
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....9ca07966d863d1f15cb86297179c071d