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In-line Fluorescence Spectroscopy for Quantification of Low Amount of Active Pharmaceutical Ingredient
- Source :
- Sørensen, D H, Christensen, N P A, Skibsted, E, Rantanen, J & Rinnan, Å 2022, ' In-line Fluorescence Spectroscopy for Quantification of Low Amount of Active Pharmaceutical Ingredient ', Journal of Pharmaceutical Sciences, vol. 111, no. 9, pp. 2406-2410 . https://doi.org/10.1016/j.xphs.2022.06.008
- Publication Year :
- 2022
-
Abstract
- The pharmaceutical industry is currently implementing new manufacturing principles and modernizing the related processing solutions. A key element in this development is implementation of process analytical technologies (PAT) for measuring product quality in a real-time mode, ideally for a continuously operating processing line. Near-infrared (NIR) spectroscopy is widely used for this purpose, but has limited use for low concentration formulations, due to its inherent detection limit. Light-induced fluorescence (LIF) spectroscopy is a PAT tool that can be used to quantify low concentrations of active pharmaceutical ingredient, and recent development of instrumentation has made it available for in-line applications. In this study, the content of tryptophan in a dynamic powder flow could be measured as low as 0.10 w/w % with LIF spectroscopy with good accuracy of RMSEP = 0.008 w/w %. Both partial least squares regression and support vector machines (SVM) were investigated, but we found SVM to be the better option due to non-linearities between the calibration test and the in-line measurements. With the use of SVM, LIF spectroscopy is a promising candidate for low concentration applications where NIR is not suitable.
Details
- ISSN :
- 15206017
- Volume :
- 111
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of pharmaceutical sciences
- Accession number :
- edsair.doi.dedup.....9c95bc0844c76265b638df154abe9529