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High prevalence ofSalmonellaand IMP-4-producing Enterobacteriaceae in the silver gull on Five Islands, Australia
High prevalence ofSalmonellaand IMP-4-producing Enterobacteriaceae in the silver gull on Five Islands, Australia
- Source :
- Journal of Antimicrobial Chemotherapy
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Objectives The objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing Enterobacteriaceae (CPE) in south-east Australia. Methods A total of 504 cloacal samples were collected from gull chicks at three nesting colonies in New South Wales, Australia [White Bay (n = 144), Five Islands (n = 200) and Montague Island (n = 160)] and were examined for salmonellae and CPE. Isolates were tested for carbapenemase genes and susceptibility to 14 antibiotics. Clonality was determined by PFGE and MLST. Genetic context and conjugative transfer of the carbapenemase gene were determined. Results A total of 120 CPE of 10 species, mainly Escherichia coli (n = 85), carrying the gene blaIMP-4, blaIMP-38 or blaIMP-26 were obtained from 80 (40%) gulls from Five Islands. Thirty percent of birds from this colony were colonized by salmonellae. Most isolates contained the gene within a class 1 integron showing a blaIMP-4-qacG-aacA4-catB3 array. The blaIMP gene was carried by conjugative plasmids of variable sizes (80–400 kb) and diverse replicons, including HI2-N (n = 30), HI2 (11), A/C (17), A/C-Y (2), L/M (5), I1 (1) and non-typeable (6). Despite the overall high genetic variability, common clones and plasmid types were shared by different birds and bacterial isolates, respectively. Conclusions Our data demonstrate a large-scale transmission of carbapenemase-producing bacteria into wildlife, likely as a result of the feeding habits of the birds at a local waste depot. The isolates from gulls showed significant similarities with clinical isolates from Australia, suggesting the human origin of the isolates. The sources of CPE for gulls on Five Islands should be explored and proper measures applied to stop the transmission into the environment.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
Salmonella
Genotype
030106 microbiology
Environmental pollution
Context (language use)
Microbial Sensitivity Tests
Integron
medicine.disease_cause
beta-Lactamases
Microbiology
Birds
Charadriiformes
03 medical and health sciences
Silver gull
Bacterial Proteins
Cloaca
Enterobacteriaceae
Prevalence
Pulsed-field gel electrophoresis
medicine
Animals
Humans
Pharmacology (medical)
Original Research
Islands
Pharmacology
Molecular Epidemiology
biology
Molecular epidemiology
Bird Diseases
Enterobacteriaceae Infections
biology.organism_classification
Anti-Bacterial Agents
Molecular Typing
Infectious Diseases
biology.protein
Multilocus sequence typing
New South Wales
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....9c8e6d65c4c034dda9c65241bd06bfbf
- Full Text :
- https://doi.org/10.1093/jac/dkv306