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Effect of Benzene Exposure on the Urinary Biomarkers of Nucleic Acid Oxidation in Two Cohorts of Gasoline Pump Attendants
- Source :
- International Journal of Environmental Research and Public Health, International Journal of Environmental Research and Public Health, Vol 16, Iss 1, p 129 (2019), Volume 16, Issue 1
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- (1) Background: The oxidized guanine derivatives excreted into urine, products of DNA and RNA oxidation and repair, are used as biomarkers of oxidative damage in humans. This study aims to evaluate oxidative damage in gasoline pump attendants occupationally exposed to benzene. Benzene is contained in the gasoline but it is also produced from traffic and from smoking. (2) Methods: Twenty-nine gasoline pump attendants from two major cities of Saudi Arabia and 102 from Italy were studied for urinary 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2&prime<br />deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and S-phenyl-mercapturic acid (SPMA) for benzene exposure and urinary cotinine for smoking status assessment by liquid chromatography-tandem mass spectrometry. Airborne benzene was also assessed in the Italian group by gas-chromatography with flame ionization detector (GC-FID). (3) Results: The results suggest that high levels of benzene exposure can cause an accumulation of SPMA and bring about the formation of the oxidation biomarkers studied to saturation. At low exposure levels, SPMA and oxidation biomarker levels were correlated among them and were associated with the smoking habit. (4) Conclusions: The study confirms the association between benzene exposure and the excretion of nucleic acid oxidation biomarkers and enhances the importance of measuring the smoking habit, as it can significantly influence oxidative damage, especially when the exposure levels are low.
- Subjects :
- Male
SPMA
Health, Toxicology and Mutagenesis
Saudi Arabia
lcsh:Medicine
Urine
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Article
Gas Chromatography-Mass Spectrometry
Excretion
Cohort Studies
03 medical and health sciences
chemistry.chemical_compound
Nucleic Acids
Occupational Exposure
medicine
Deoxyguanosine
oxidative stress
Humans
Food science
urinary biomarkers
Gasoline
Benzene
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
Chromatography
Liquid
Chemistry
nucleic acids oxidation
lcsh:R
Public Health, Environmental and Occupational Health
Benzene exposure
Nucleic acids oxidation
Oxidative stress
Spma
Urinary biomarkers
Acetylcysteine
Biomarkers
Chromatography, Liquid
Italy
Oxidation-Reduction
benzene exposure
Nucleic acid
Cotinine
Subjects
Details
- Language :
- English
- ISSN :
- 16604601 and 16617827
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....9c6932a141a0fdd23bc9373f41780051