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Effects of polyunsaturated fatty acids on the development of pig oocytes in vitro following parthenogenetic activation and on the lipid content of oocytes and embryos
- Source :
- Animal reproduction science. 205
- Publication Year :
- 2019
-
Abstract
- As oocytes and embryos of pigs have greater lipid content in the cytoplasm than those of other species, supplementation of the medium for in vitro maturation (IVM) of oocytes with omega-3 polyunsaturated fatty acids (PUFA) may help to improve embryo development. This study was conducted to evaluate effects of the inclusion of the docosaexaenoic (DHA) and of the eicosapentaenoic acids (EPA) in the IVM medium on the development of pig oocytes and on the lipid content of oocytes and embryos. In all experiments, control media consisted of porcine follicular fluid and oocytes were activated through parthenogenesis. In Experiment 1, there were four treatments for each PUFA: one control; and three treatments including EPA or DHA in the IVM medium at 12.5 μM, 25.0 μM and 50.0 μM). In Experiment 2, inclusion of 50 μM DHA was compared against the control. Cleavage rates in the IVM medium including 12.5 μM EPA and blastocyst development rates in media at any EPA concentration were less than for the control in Experiment 1 (P 0.05). Compared to the control, inclusion of 50 μM DHA in the IVM medium was related to greater cleavage rates and greater number of embryo cells, in Experiment 1, and lesser lipid content in oocytes after 22 and 44 h and in embryos after 7 days, in Experiment 2 (both P 0.05). Addition of DHA in the IVM medium may benefit the development of pig oocytes, but EPA appears to be cytotoxic.
- Subjects :
- Docosahexaenoic Acids
Swine
Parthenogenesis
Andrology
03 medical and health sciences
0302 clinical medicine
Endocrinology
Food Animals
medicine
Animals
Blastocyst
chemistry.chemical_classification
030219 obstetrics & reproductive medicine
Dose-Response Relationship, Drug
Embryogenesis
0402 animal and dairy science
Embryo
04 agricultural and veterinary sciences
General Medicine
Embryo, Mammalian
Lipid Metabolism
040201 dairy & animal science
Follicular fluid
In vitro
In vitro maturation
In Vitro Oocyte Maturation Techniques
medicine.anatomical_structure
chemistry
Eicosapentaenoic Acid
embryonic structures
Oocytes
lipids (amino acids, peptides, and proteins)
Animal Science and Zoology
Female
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 18732232
- Volume :
- 205
- Database :
- OpenAIRE
- Journal :
- Animal reproduction science
- Accession number :
- edsair.doi.dedup.....9c63395440c7c76cfa1b4630914b3a70