Back to Search
Start Over
Determination of the reactivities and cross-reactivities of monoclonal antibodies against staphylococcal enterotoxin A by indirect ELISA and immunoblot including a semiautomated electrophoresis system
Determination of the reactivities and cross-reactivities of monoclonal antibodies against staphylococcal enterotoxin A by indirect ELISA and immunoblot including a semiautomated electrophoresis system
- Source :
- Scopus-Elsevier
- Publication Year :
- 1992
- Publisher :
- Oxford University Press (OUP), 1992.
-
Abstract
- Eight murine monoclonal antibodies (Mabs) to staphylococcal enterotoxin A (SEA) were produced using standard hybridoma techniques. We studied reactivities and cross-reactivities by indirect ELISA and immunoblotting. Two of these Mabs (A5 and A7) reacted with five serovars (A-E) of SE in both systems. Only Mab A1 reacted specifically with the homologous toxin, while four Mabs reacted with SEA and SEE. Mabs A5 and A7 could be used to detect all five serovars of SEs in a single assay.
- Subjects :
- Serotype
Staphylococcus aureus
medicine.drug_class
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Enterotoxin
Cross Reactions
Monoclonal antibody
medicine.disease_cause
Applied Microbiology and Biotechnology
Enterotoxins
Mice
medicine
Animals
Polyacrylamide gel electrophoresis
Mice, Inbred BALB C
Hybridomas
biology
Chemistry
Toxin
Antibodies, Monoclonal
Molecular biology
Blot
Immunoglobulin G
biology.protein
Electrophoresis, Polyacrylamide Gel
Female
Antibody
Subjects
Details
- ISSN :
- 1472765X and 02668254
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Letters in Applied Microbiology
- Accession number :
- edsair.doi.dedup.....9c5fff50cc82d952acf816670c570721
- Full Text :
- https://doi.org/10.1111/j.1472-765x.1992.tb00689.x