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Determination of the reactivities and cross-reactivities of monoclonal antibodies against staphylococcal enterotoxin A by indirect ELISA and immunoblot including a semiautomated electrophoresis system

Determination of the reactivities and cross-reactivities of monoclonal antibodies against staphylococcal enterotoxin A by indirect ELISA and immunoblot including a semiautomated electrophoresis system

Authors :
Esperanza Gomez-Lucia
José L. Blanco
José A. Orden
Guillermo Suárez
Ana Doménech
Javier Hernández
Joaquín Goyache
Source :
Scopus-Elsevier
Publication Year :
1992
Publisher :
Oxford University Press (OUP), 1992.

Abstract

Eight murine monoclonal antibodies (Mabs) to staphylococcal enterotoxin A (SEA) were produced using standard hybridoma techniques. We studied reactivities and cross-reactivities by indirect ELISA and immunoblotting. Two of these Mabs (A5 and A7) reacted with five serovars (A-E) of SE in both systems. Only Mab A1 reacted specifically with the homologous toxin, while four Mabs reacted with SEA and SEE. Mabs A5 and A7 could be used to detect all five serovars of SEs in a single assay.

Details

ISSN :
1472765X and 02668254
Volume :
14
Database :
OpenAIRE
Journal :
Letters in Applied Microbiology
Accession number :
edsair.doi.dedup.....9c5fff50cc82d952acf816670c570721
Full Text :
https://doi.org/10.1111/j.1472-765x.1992.tb00689.x