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Binding of 5α-dihydroprogesterone to proteins in human pregnancy serum
- Source :
- Acta Endocrinologica. 100:120-130
- Publication Year :
- 1982
- Publisher :
- Oxford University Press (OUP), 1982.
-
Abstract
- The binding of 5α-dihydroprogesterone (DHP)1 to proteins in pregnancy serum has been investigated and compared with the binding of progesterone. Characteristic properties of the DHP-serum protein interaction were unsaturability, low affinity and poor complex stability. Fractionation of serum using a variety of protein separation techniques, revealed that DHP interacts with several proteins. At low temperature (0–4°C) albumin appeared to be the principal binding component whereas higher temperatures seemed to favour binding to β-lipoproteins and α2-macroglobulin. Binding to specific binding proteins such as the corticosteroid binding globulin (CBG) and the sex hormone-binding globulin, (SHBG) were detectable but appeared to be quantitatively unimportant. Progesterone showed a similar multicomponent interaction but differed from DHP in the extent of binding to CBG. Binding of either hormone to the α1-acid-glycoprotein was negligible. The present study shows that the high endogenous DHP levels present in pregnancy sera are caused by factors other than high affinity protein binding.
- Subjects :
- medicine.medical_specialty
Globulin
Endocrinology, Diabetes and Metabolism
Plasma protein binding
Pregnancy Proteins
Chromatography, Affinity
Pregnanediones
chemistry.chemical_compound
Endocrinology
Sex hormone-binding globulin
Transcortin
Pregnancy
Sex Hormone-Binding Globulin
Internal medicine
Protein purification
medicine
Humans
alpha-Macroglobulins
Progesterone
biology
Chemistry
Albumin
5-alpha-Dihydroprogesterone
5α-Dihydroprogesterone
Blood Proteins
Orosomucoid
General Medicine
Blood proteins
Lipoproteins, LDL
Chromatography, Gel
biology.protein
Electrophoresis, Polyacrylamide Gel
Female
Isoelectric Focusing
Protein Binding
Subjects
Details
- ISSN :
- 1479683X and 08044643
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Acta Endocrinologica
- Accession number :
- edsair.doi.dedup.....9c5fb865ddb68e98e124698b9540b13f