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The anticarcinogen activity of β-arbutin on MCF-7 cells: Stimulation of apoptosis through estrogen receptor-α signal pathway, inflammation and genotoxicity
- Source :
- Molecular and Cellular Biochemistry. 476:349-360
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Arbutin is one of the active ingredients employed in cosmetics as a skin whitening agent. In the present study, the possible effects of arbutin on breast cancer were determined with human breast adenocarcinoma (MCF-7) cells. α and β-arbutin cytotoxicity levels in MCF-7 cells were determined with the MTT method. At low (1–10 mM) doses, α-arbutin appears to be more toxic than β-arbutin. At higher (5–200 mM) and LD50 doses beta arbutin toxicity appears to be higher than alpha arbutin. Thus, the study was continued with β -arbutin. The effects of low and high doses of β-arbutin was determined on oxidative stress, genotoxicity, inflammation, apoptosis, proliferation, endoplasmic reticulum stress and estrogen receptor-α in MCF-7 cells. The results demonstrated that the β-arbutin doses administered to MCF-7 cells did not affect oxidative and endoplasmic reticulum stress in the experimental groups. However, it was found that administration of LD50 dose β-arbutin induced inflammation in these cells via proinflammatory cytokine levels (TNF-α, IFN-γ and IL-1β). It was observed that LD10 and LD50 doses of β-arbutin increased genotoxicity in MCF-7 cells. The gene expression analysis conducted with RT-PCR device and immunocytochemical analysis revealed that β-arbutin at LD50 dose induced apoptosis in MCF-7 cells via p53 and Caspase 3. Furthermore, it was determined that all β-arbutin doses inhibited estrogen receptor-α in MCF-7 cells. Considering that arbutin increased the activation of apoptotic Caspase 3 through p53, which was stimulated by genotoxic and inflammatory effects at LD50 dose in MCF-7 cells. Determination of this mechanism behind these effects of β-arbutin may contribute to the development of a new perspective in treatment.
- Subjects :
- 0301 basic medicine
Cell Survival
Clinical Biochemistry
Estrogen receptor
Apoptosis
Breast Neoplasms
Inflammation
Caspase 3
Pharmacology
medicine.disease_cause
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
medicine
Anticarcinogenic Agents
Humans
Molecular Biology
Anticarcinogen
Cell Proliferation
Micronucleus Tests
Arbutin
Estrogen Receptor alpha
Cell Biology
General Medicine
Endoplasmic Reticulum Stress
Oxidative Stress
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
MCF-7 Cells
Female
Comet Assay
medicine.symptom
Genotoxicity
DNA Damage
Mutagens
Signal Transduction
Subjects
Details
- ISSN :
- 15734919 and 03008177
- Volume :
- 476
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....9c411d0de3dd8aa5511ea3fa17d7ea65