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Solubilization of human serotonin1A receptors expressed in neuronal cells

Authors :
Amitabha Chattopadhyay
Md. Jafurulla
Source :
Chemistry and Physics of Lipids. 150:244-249
Publication Year :
2007
Publisher :
Elsevier BV, 2007.

Abstract

The serotonin 1A receptor is an important member of the G-protein coupled receptor family, and is involved in a variety of cognitive, behavioral, and developmental functions. None of the subtypes of G-protein coupled serotonin receptors have yet been purified to homogeneity from natural sources. We report here, for the first time, the solubilization of human serotonin 1A receptors stably expressed in neuronal (HN2) cells. Importantly, ligand binding assay shows that the serotonin 1A receptor solubilized this way is functionally active. The effective solubilization of the serotonin 1A receptor from neuronal cells represents an important step toward the purification of the receptor in native-like membrane environment.

Details

ISSN :
00093084
Volume :
150
Database :
OpenAIRE
Journal :
Chemistry and Physics of Lipids
Accession number :
edsair.doi.dedup.....9c39e97d95148fe66507eec4dfde6e1e
Full Text :
https://doi.org/10.1016/j.chemphyslip.2007.08.003